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Typical Testing Data

Mouse Early Growth Response Protein 1 ELISA Kit | EGR1 elisa kit

Mouse Early Growth Response Protein 1 ELISA Kit

Gene Names
EGR1; TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268
Reactivity
Mouse
Synonyms
Early Growth Response Protein 1; Mouse Early Growth Response Protein 1 ELISA Kit; EGR1/KROX24/NGFIA/ZNF225/AT225Transcription factor ETR103/early growth response 1/early growth response protein 1/EGR-1/G0S30/KROX24/KROX-24/Nerve growth factor-induced protein A/NGFI-ATranscription factor Zif268/TIS8/ZIF-268/Zinc finger protein Krox-24/ZNF225Zinc finger protein 225; EGR1 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Sequence Length
543
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Testing Data

Typical Standard Curve

Typical Standard Curve
Related Product Information for EGR1 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse EGR1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse EGR1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse EGR1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse EGR1 can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,507 Da
NCBI Official Full Name
early growth response protein 1
NCBI Official Synonym Full Names
early growth response 1
NCBI Official Symbol
EGR1
NCBI Official Synonym Symbols
TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268
NCBI Protein Information
early growth response protein 1
UniProt Protein Name
Early growth response protein 1
UniProt Gene Name
EGR1
UniProt Synonym Gene Names
KROX24; ZNF225; EGR-1; NGFI-A
UniProt Entry Name
EGR1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene. [provided by RefSeq, Dec 2014]

Uniprot Description

EGR1: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. By growth factors. Belongs to the EGR C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: DNA binding; histone acetyltransferase binding; metal ion binding; protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: BMP signaling pathway; cytokine and chemokine mediated signaling pathway; negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of protein sumoylation; response to glucose stimulus; response to insulin stimulus; T cell differentiation; transcription from RNA polymerase II promoter

Research Articles on EGR1

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Product Notes

The Mouse EGR1 egr1 (Catalog #AAA763888) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA763888 ELISA Kit recognizes Mouse EGR1. It is sometimes possible for the material contained within the vial of "Early Growth Response Protein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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