Rabbit anti-Human, Mouse Zic1 Polyclonal Antibody | anti-ZIC1 antibody

Zic1 (Zinc Finger Protein of the Cerebellum 1, Zic Family Member 1 (Odd-paired Drosophila Homolog), Zic Family Member 1, Zic 1, Zic-1, ZIC, Odd Paired Homolog Drosophila, Zinc Finger Protein ZIC 1, Zinc Finger Protein ZIC1, ZNF201)

Cat. Num. AAA623141

• Gene Names: ZIC1; ZIC; ZNF201
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Purified; Purified
• Synonyms: Zic1; Polyclonal Antibody; Zic1 (Zinc Finger Protein of the Cerebellum 1; Zic Family Member 1 (Odd-paired Drosophila Homolog); Zic Family Member 1; Zic 1; Zic-1; ZIC; Odd Paired Homolog Drosophila; Zinc Finger Protein ZIC 1; Zinc Finger Protein ZIC1; ZNF201); Anti -Zic1 (Zinc Finger Protein of the Cerebellum 1; anti-ZIC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects a band of approximately 60kD. Species Crossreactivity: Crossreacts with Human and Mouse.
• Purity/Purification: Purified; Purified
• Form/Format: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7

• Applicable Applications for anti-ZIC1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
• Immunogen: Synthetic peptide - which corresponding to amino acids 25-42 of mouse Zic-1. This domain is completely conserved in human ZIC-1.
• Cellular Localization: Nuclear
• Positive Control: Mouse cerebellum
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 3 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ZIC1 antibody

The Zic-1 gene, which encodes a zinc finger protein, is expressed in the developing or matured central nervous system in a highly restricted manner. The Zic gene is expressed in granule cells that make synaptic contact with Purkinje cells. Clearly Zic-1 is a gene critical to cerebellar pattern formation. The expression of Zic genes is first detected at gastrulation and at neurulation, becomes restricted to the dorsal neural ectoderm and the dorsal paraxial mesoderm. Zic-2 and Zic-3 are highly similar genes, especially in their product's zinc finger motif and by comparison of their genomic organization in that they share common exon-intron boundaries and belong to the same gene family. By comparison in function, Zic-2 is essential for the formation of the brain and Zic-3 is important for right and left axis formation. The Zic-1 gene has been mapped to chromosome 9 in mouse. The 5' flanking region of the Zic-1 gene contains a region-specific enhancer determined to be essential in in vivo and in vitro deletion analysis. The temporal profile of mRNA expression differs for each of the Zic gene products. The Drosophila odd-paired gene is highly homologous to the Zic gene family.

Product Categories/Family for anti-ZIC1 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 22547182
• NCBI GeneID: 7545
• NCBI Accession #: NP_003403.2
• NCBI GenBank Nucleotide #: NM_003412.3
• UniProt Accession #: Q15915; Q2M3N1
• Molecular Weight: 48,309 Da
• NCBI Official Full Name: zinc finger protein ZIC 1
• NCBI Official Synonym Full Names: Zic family member 1
• NCBI Official Symbol: ZIC1
• NCBI Official Synonym Symbols: ZIC; ZNF201
• NCBI Protein Information: zinc finger protein ZIC 1; OTTHUMP00000215402; zinc finger protein 201; Zinc finger protein of the cerebellum 1; Zic family member 1 (odd-paired homolog, Drosophila)
• UniProt Protein Name: Zinc finger protein ZIC 1
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZIC1
• UniProt Synonym Gene Names: ZIC; ZNF201
• UniProt Entry Name: ZIC1_HUMAN

NCBI Description

This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development. Aberrant expression of this gene is seen in medulloblastoma, a childhood brain tumor. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 4, a related family member on chromosome 3. This gene encodes a transcription factor that can bind and transactivate the apolipoprotein E gene. [provided by RefSeq]

Uniprot Description

ZIC1: Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally- located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'- TGGGTGGTC-3'. Belongs to the GLI C2H2-type zinc-finger protein family.

Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: DNA binding; metal ion binding; transcription factor activity

Biological Process: inner ear morphogenesis; positive regulation of protein import into nucleus; transcription, DNA-dependent; spinal cord development; positive regulation of transcription, DNA-dependent; brain development; cell differentiation; adult walking behavior; pattern specification process; regulation of smoothened signaling pathway