Immunohistochemistry (IHC)
(MBS9603735 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit ZFYVE16 Polyclonal Antibody | anti-ZFYVE16 antibody
ZFYVE16 Antibody
Gene Names
ZFYVE16; PPP1R69
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ZFYVE16; Polyclonal Antibody; ZFYVE16 Antibody; AI035632; B130024H06Rik; B130031L15; DKFZp686E13162; Endofin; Endosomal associated FYVE domain protein; Endosome associated FYVE domain protein; Endosome-associated FYVE domain protein; KIAA0305; mKIAA0305; OTTMUSP00000029589; RGD1564784; ZFY16_HUMAN; Zinc finger FYVE domain containing protein 16; Zinc finger FYVE domain-containing protein 16; Zinc finger; FYVE domain containing 16; anti-ZFYVE16 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ZFYVE16 antibody detects endogenous levels of ZFYVE16.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
809
Applicable Applications for anti-ZFYVE16 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human ZFYVE16.
Subcellular Location
Cytoplasm. Early Endosome Membrane. Localized to early endosomes. Membrane-associated, probably via its association with phosphatidylinositol 3-phosphate.
Tissue Specificity
Widely expressed. Highly expressed in kidney, placenta and lung. Expressed at intermediate level in heart, brain, skeletal muscle, spleen and liver. Weakly expressed in colon, thymus and peripheral blood lymphocytes.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Dog, Chicken
Similarity
Bovine (92%), Horse (85%), Sheep (92%), Dog (85%), Chicken (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9603735 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603735 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of HEK-293 cells, using ZFYVE16 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of HEK-293 cells, using ZFYVE16 antibody.)
Related Product Information for anti-ZFYVE16 antibody
Function: May be involved in regulating membrane trafficking in the endosomal pathway. Overexpression induces endosome aggregation. Required to target TOM1 to endosomes.
Subunit Structure: Interacts with the C-terminus of TOM1. Does not interact with TOM1L1 or TOM1L2.
Similarity: The FYVE-type zinc finger is necessary and sufficient for its localization into early endosomes and mediates the association with PI3P.
Subunit Structure: Interacts with the C-terminus of TOM1. Does not interact with TOM1L1 or TOM1L2.
Similarity: The FYVE-type zinc finger is necessary and sufficient for its localization into early endosomes and mediates the association with PI3P.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 169 kDa; 250 kDa
Predicted: 169 kDa
Predicted: 169 kDa
NCBI Official Full Name
zinc finger FYVE domain-containing protein 16 isoform b
NCBI Official Synonym Full Names
zinc finger FYVE-type containing 16
NCBI Official Symbol
ZFYVE16
NCBI Official Synonym Symbols
PPP1R69
NCBI Protein Information
zinc finger FYVE domain-containing protein 16
UniProt Protein Name
Zinc finger FYVE domain-containing protein 16
Protein Family
UniProt Gene Name
ZFYVE16
UniProt Synonym Gene Names
KIAA0305
NCBI Description
This gene encodes an endosomal protein that belongs to the FYVE zinc finger family of proteins. The encoded protein is thought to regulate membrane trafficking in the endosome. This protein functions as a scaffold protein in the transforming growth factor-beta signaling pathway and is involved in positive and negative feedback regulation of the bone morphogenetic protein signaling pathway. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
Uniprot Description
May be involved in regulating membrane trafficking in the endosomal pathway. Overexpression induces endosome aggregation. Required to target TOM1 to endosomes.
Research Articles on ZFYVE16
Similar Products
Product Notes
The ZFYVE16 zfyve16 (Catalog #AAA9603735) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZFYVE16 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ZFYVE16 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the ZFYVE16 zfyve16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZFYVE16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
