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Western Blot (WB) (Western blotAll lanes: CSPG4 antibody IgG at 2.51ug/ml+ A375 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 251 kDaObserved band size: 251 kDa)

Rabbit anti-Human CSPG4 Polyclonal Antibody | anti-CSPG4 antibody

CSPG4 Antibody

Gene Names
CSPG4; NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Synonyms
CSPG4; Polyclonal Antibody; CSPG4 Antibody; Chondroitin sulfate proteoglycan 4; Chondroitin sulfate proteoglycan NG2; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan; MCSP; anti-CSPG4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Sequence Length
2322
Applicable Applications for anti-CSPG4 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Species
Human
Immunogen
Recombinant human Chondroitin sulfate proteoglycan 4 protein (1950-2210AA)
Conjugate
Non-conjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-30921 / sc-30922 / sc-30923 / sc-20162
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: CSPG4 antibody IgG at 2.51ug/ml+ A375 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 251 kDaObserved band size: 251 kDa)

Western Blot (WB) (Western blotAll lanes: CSPG4 antibody IgG at 2.51ug/ml+ A375 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 251 kDaObserved band size: 251 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer MBS7046373 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver cancer MBS7046373 at dilution 1:100)
Related Product Information for anti-CSPG4 antibody
Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
250,537 Da
NCBI Official Full Name
chondroitin sulfate proteoglycan 4
NCBI Official Synonym Full Names
chondroitin sulfate proteoglycan 4
NCBI Official Symbol
CSPG4
NCBI Official Synonym Symbols
NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG
NCBI Protein Information
chondroitin sulfate proteoglycan 4
UniProt Protein Name
Chondroitin sulfate proteoglycan 4
UniProt Gene Name
CSPG4
UniProt Synonym Gene Names
MCSP
UniProt Entry Name
CSPG4_HUMAN

NCBI Description

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]

Uniprot Description

NG2: a transmembrane chondrotin sulfate proteoglycan expressed on several types of immature progenitor cells and human malignant melanoma cells. Plays a role in cell proliferation and motility of endothelial cells during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. May regulate MPP16-dependent degradation and invasion of type I collagen participating to melanoma cells invasion properties. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through Cdc42, ACK1 and P130Cas. May activate FAK and ERK signaling cascades. PKC-alpha-mediated NG2 phosphorylation may be a key step for initiating cell polarization and motility.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: cell surface; extracellular region; focal adhesion; Golgi lumen; integral to plasma membrane; lysosomal lumen

Molecular Function: protein kinase binding

Biological Process: cell migration; chondroitin sulfate biosynthetic process; chondroitin sulfate catabolic process; dermatan sulfate biosynthetic process; glycosaminoglycan metabolic process; positive regulation of peptidyl-tyrosine phosphorylation

Research Articles on CSPG4

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Product Notes

The CSPG4 cspg4 (Catalog #AAA7046373) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CSPG4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CSPG4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the CSPG4 cspg4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CSPG4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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