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Western Blot (WB) (Western blotAll lanes: ZDHHC6 antibody at 0.2ug/mlLane 1: NIH/3T3 whole cell lysateLane 2: K562 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 48 kDaObserved band size: 48 kDa)

Rabbit anti-Human, Mouse ZDHHC6 Polyclonal Antibody | anti-ZDHHC6 antibody

ZDHHC6 Antibody

Gene Names
ZDHHC6; DHHC-6; ZNF376
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antigen Affinity Purified
Synonyms
ZDHHC6; Polyclonal Antibody; ZDHHC6 Antibody; Palmitoyltransferase ZDHHC6 (EC 2.3.1.225) (Transmembrane protein H4) (Zinc finger DHHC domain-containing protein 6) (DHHC-6) (Zinc finger protein 376); ZNF376; anti-ZDHHC6 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Sequence Length
409
Applicable Applications for anti-ZDHHC6 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200
Immunogen
Recombinant Human Palmitoyltransferase ZDHHC6 protein (227-409AA)
Conjugate
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Species
Human
Research Area
Cell Biology
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: ZDHHC6 antibody at 0.2ug/mlLane 1: NIH/3T3 whole cell lysateLane 2: K562 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 48 kDaObserved band size: 48 kDa)

Western Blot (WB) (Western blotAll lanes: ZDHHC6 antibody at 0.2ug/mlLane 1: NIH/3T3 whole cell lysateLane 2: K562 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 48 kDaObserved band size: 48 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human skin tissue using MBS1499700 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human skin tissue using MBS1499700 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS1499700 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney tissue using MBS1499700 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of NIH3T3 cells using MBS1499700 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunofluorescence (IF) (Immunofluorescent analysis of NIH3T3 cells using MBS1499700 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-ZDHHC6 antibody
Palmitoylates calnexin (CALX), which is required for its association with the ribosome-translocon complex and efficient folding of glycosylated proteins.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
palmitoyltransferase ZDHHC6 isoform 2
NCBI Official Synonym Full Names
zinc finger, DHHC-type containing 6
NCBI Official Symbol
ZDHHC6
NCBI Official Synonym Symbols
DHHC-6; ZNF376
NCBI Protein Information
palmitoyltransferase ZDHHC6
UniProt Protein Name
Palmitoyltransferase ZDHHC6
Protein Family
UniProt Gene Name
ZDHHC6
UniProt Synonym Gene Names
ZNF376; DHHC-6
UniProt Entry Name
ZDHC6_HUMAN

Uniprot Description

ZDHHC6: Palmitoylates calnexin (CALX), which is required for its association with the ribosome-translocon complex and efficient folding of glycosylated proteins. Belongs to the DHHC palmitoyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transferase; EC 2.3.1.225; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 10q25.2

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane

Molecular Function: palmitoyltransferase activity; protein-cysteine S-palmitoleyltransferase activity; zinc ion binding

Biological Process: protein palmitoylation

Research Articles on ZDHHC6

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Product Notes

The ZDHHC6 zdhhc6 (Catalog #AAA1499700) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZDHHC6 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ZDHHC6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200. Researchers should empirically determine the suitability of the ZDHHC6 zdhhc6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZDHHC6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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