Western Blot (WB)
(Western blot analysis of UBE2B expression in Jurkat (A), NIH3T3 (B), mouse heart (C), rat heart (D), rat brain (E) whole cell lysates.)
Rabbit UBE2B Polyclonal Antibody | anti-UBE2B antibody
Anti-UBE2B Antibody
Western Blot (WB)
(Western blot analysis of UBE2B expression in Jurkat (A), NIH3T3 (B), mouse heart (C), rat heart (D), rat brain (E) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of UBE2B expression in Jurkat (A), NIH3T3 (B), mouse heart (C), rat heart (D), rat brain (E) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of UBE2B staining in rat lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of UBE2B staining in rat lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008]
Uniprot Description
UBE2B: a ubiquitin-protein ligase, a member of the ubiquitin-conjugating enzyme family. Homologous to the yeast DNA repair gene RAD6. Interacts with RAD18. Required for postreplication repair of UV-damaged DNA.
Protein type: EC 6.3.2.19; Ubiquitin ligase; Ligase; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: chromatin; cytoplasm; nuclear chromatin; nucleoplasm; nucleus; plasma membrane; replication fork; XY body
Molecular Function: ATP binding; protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity
Biological Process: chiasma formation; DNA damage response, detection of DNA damage; DNA repair; histone H2A ubiquitination; in utero embryonic development; maintenance of chromatin silencing; negative regulation of apoptosis; negative regulation of histone phosphorylation; postreplication repair; proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; protein monoubiquitination; protein polyubiquitination; protein stabilization; protein ubiquitination; response to DNA damage stimulus; response to drug; response to UV; sperm axoneme assembly; spermatogenesis; telomere clustering; ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway through beta-catenin
Research Articles on UBE2B
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Product Notes
The UBE2B ube2b (Catalog #AAA8245695) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-UBE2B Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1/500 - 1/2000; IHC: 1/50 - 1/200. Researchers should empirically determine the suitability of the UBE2B ube2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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