Western Blot (WB)
(Anti-ZDHHC3 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:48 kDa)
Rabbit anti-Human ZDHHC3 Polyclonal Antibody | anti-ZDHHC3 antibody
Anti-ZDHHC3 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
ZDHHC3; Polyclonal Antibody; Anti-ZDHHC3 Antibody; Rabbit Polyclonal; ZDHHC3 Antibody; Rabbit PAb; Antigen Affinity Purified; zinc finger DHHC-type containing 3; Anti-DHHC-3 Antibody; Anti-DHHC3 Antibody; Anti-GODZ Antibody; Anti-ZNF373 Antibody; anti-ZDHHC3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ZDHHC3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-ZDHHC3 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
E Coli-derived Human ZDHHC3 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human ZDHHC3 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-ZDHHC3 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:48 kDa)
Western Blot (WB)
(Anti-ZDHHC3 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:48 kDa)
Related Product Information for anti-ZDHHC3 antibody
ZDHHC3, also known as GODZ (Golgi apparatus-specific DHHC zinc finger protein), has been shown to palmitoylate AMPA, NMDA, and GABAA receptors and hence to control their trafficking at the synapses. Besides, ZDHHC3 can palmitoylate a number of proteins involved in control of neuronal morphogenesis, such as the alpha6 integrin subunit, Galphas and Galphaq proteins, and stathmins 2 and 3. ZDHHC3 utilizes a "ping-pong" kinetic mechanism to transfer palmitate to the target protein, while no regulatory sites on the ZDHHC3 protein have yet been discovered. ZDHHC3 tyrosine residues are specific targets for FGFR1 and Src kinases. DHHC3 is upregulated in malignant and metastatic human breast cancer. Elevated expression of DHHC3 correlated with diminished patient survival in breast cancer and other human cancer types.
References
Sharma C, et al. (2017) Protein acyltransferase dhhc3 regulates breast tumor growth, oxidative stress, and senescence. Cancer Res 77 (24): 6880-6890.Lievens PM, et al. (2016) Zdhhc3 tyrosine phosphorylation regulates neural cell adhesion molecule palmitoylation. Mol Cell Biol 36 (17): 2208-2225.Kinney N, et al. (2017) Zdhhc3 as a risk and mortality marker for breast cancer in african american women. Cancer Inform 16 1176935117746644.
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Product Notes
The ZDHHC3 (Catalog #AAA8109400) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ZDHHC3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZDHHC3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ZDHHC3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZDHHC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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