Western Blot (WB)
(ZC4H2 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the ZC4H2 antibody detected the ZC4H2 protein (arrow).)
Rabbit anti-Human ZC4H2 Polyclonal Antibody | anti-ZC4H2 antibody
ZC4H2, CT (ZC4H2, HCA127, KIAA1166, Zinc finger C4H2 domain-containing protein, Hepatocellular carcinoma-associated antigen 127) (FITC)
Gene Names
ZC4H2; MCS; WWS; WRWF; MRXS4; HCA127; WRWFFR; KIAA1166
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ZC4H2; Polyclonal Antibody; CT (ZC4H2; HCA127; KIAA1166; Zinc finger C4H2 domain-containing protein; Hepatocellular carcinoma-associated antigen 127) (FITC); Hepatocellular carcinoma-associated antigen 127; anti-ZC4H2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Sequence Length
224
Applicable Applications for anti-ZC4H2 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ZC4H2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-228 amino acids from the C-terminal region of human ZC4H2.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(ZC4H2 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the ZC4H2 antibody detected the ZC4H2 protein (arrow).)
Western Blot (WB)
(ZC4H2 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the ZC4H2 antibody detected the ZC4H2 protein (arrow).)
Immunohistochemistry (IHC)
(ZC4H2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZC4H2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ZC4H2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZC4H2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(ZC4H2 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ZC4H2 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ZC4H2 antibody
This protein contains a C4H2-type zinc finger and is thought to be involved in zinc ion binding. There are 3 isoforms produced by alternative splicing.
Product Categories/Family for anti-ZC4H2 antibody
References
Wu, C., et al. Proteomics 7(11):1775-1785(2007). Ross, M.T., et al. Nature 434(7031):325-337(2005). Wang, Y., et al. J. Immunol. 169(2):1102-1109(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
zinc finger C4H2 domain-containing protein isoform 1
NCBI Official Synonym Full Names
zinc finger C4H2-type containing
NCBI Official Symbol
ZC4H2
NCBI Official Synonym Symbols
MCS; WWS; WRWF; MRXS4; HCA127; WRWFFR; KIAA1166
NCBI Protein Information
zinc finger C4H2 domain-containing protein
UniProt Protein Name
Zinc finger C4H2 domain-containing protein
Protein Family
UniProt Gene Name
ZC4H2
UniProt Synonym Gene Names
HCA127; KIAA1166
UniProt Entry Name
ZC4H2_HUMAN
NCBI Description
This gene encodes a member of the zinc finger domain-containing protein family. This family member has a C-terminal zinc finger domain that is characterized by four cysteine residues and two histidine residues, and it also includes a coiled-coil region. This protein has been detected as an autoantigen in hepatocellular carcinoma patients. This gene has been identified as a potential candidate for X-linked cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
Research Articles on ZC4H2
Similar Products
Product Notes
The ZC4H2 zc4h2 (Catalog #AAA6364878) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZC4H2, CT (ZC4H2, HCA127, KIAA1166, Zinc finger C4H2 domain-containing protein, Hepatocellular carcinoma-associated antigen 127) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZC4H2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ZC4H2 zc4h2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZC4H2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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