Rabbit anti-Human ZC3HAV1 Polyclonal Antibody | anti-ZC3HAV1 antibody

ZC3HAV1, CT (ZC3HAV1, ZC3HDC2, Zinc finger CCCH-type antiviral protein 1, ADP-ribosyltransferase diphtheria toxin-like 13, Zinc finger CCCH domain-containing protein 2, Zinc finger antiviral protein) (PE)

Cat. Num. AAA6364863

• Gene Names: ZC3HAV1; ZAP; ZC3H2; ARTD13; PARP13; FLB6421; ZC3HDC2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ZC3HAV1; Polyclonal Antibody; CT (ZC3HAV1; ZC3HDC2; Zinc finger CCCH-type antiviral protein 1; ADP-ribosyltransferase diphtheria toxin-like 13; Zinc finger CCCH domain-containing protein 2; Zinc finger antiviral protein) (PE); Zinc finger antiviral protein; anti-ZC3HAV1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ZC3HAV1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ZC3HAV1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 611-640 amino acids from the C-terminal region of human ZC3HAV1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ZC3HAV1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ZC3HAV1 antibody detected the ZC3HAV1 protein (arrow).)

Immunohistochemistry (IHC)

(ZC3HAV1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZC3HAV1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-ZC3HAV1 antibody

ZC3HAV1 is a CCCH-type zinc finger protein that is thought to prevent infection by retroviruses. Studies of the rat homolog indicate that the protein may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection.

Product Categories/Family for anti-ZC3HAV1 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004). Bick, M.J., et al. J. Virol. 77(21):11555-11562(2003). Katoh, M., et al. Int. J. Oncol. 23(2):541-547(2003). Gao, G., et al. Science 297(5587):1703-1706(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 27477136
• NCBI GeneID: 56829
• NCBI Accession #: NP_064504.2
• NCBI GenBank Nucleotide #: NM_020119.3
• UniProt Accession #: Q7Z2W4; Q8IW57; Q8TAJ3; Q96N79; Q9H8R9; Q9P0Y7; A4D1R2; A4D1S4
• Molecular Weight: 101,431 Da
• NCBI Official Full Name: zinc finger CCCH-type antiviral protein 1 isoform 1
• NCBI Official Synonym Full Names: zinc finger CCCH-type, antiviral 1
• NCBI Official Symbol: ZC3HAV1
• NCBI Official Synonym Symbols: ZAP; ZC3H2; ARTD13; PARP13; FLB6421; ZC3HDC2
• NCBI Protein Information: zinc finger CCCH-type antiviral protein 1; zinc finger CCCH domain-containing protein 2; ADP-ribosyltransferase diphtheria toxin-like 13
• UniProt Protein Name: Zinc finger CCCH-type antiviral protein 1
• Protein Family: Zinc finger CCCH-type antiviral protein
• UniProt Gene Name: ZC3HAV1
• UniProt Synonym Gene Names: ZC3HDC2; ARTD13; ZAP
• UniProt Entry Name: ZCCHV_HUMAN

Uniprot Description

ZAP: Antiviral protein which inhibits the replication of viruses by recruiting the cellular RNA degradation machineries to degrade the viral mRNAs. Binds to a ZAP-responsive element (ZRE) present in the target viral mRNA, recruits cellular poly(A)- specific ribonuclease PARN to remove the poly(A) tail, and the 3'- 5' exoribonuclease complex exosome to degrade the RNA body from the 3'-end. It also recruits the decapping complex DCP1-DCP2 through RNA helicase p72 (DDX17) to remove the cap structure of the viral mRNA to initiate its degradation from the 5'-end. Its target viruses belong to families which include retroviridae: human immunodeficiency virus type 1 (HIV-1), moloney and murine leukemia virus (MoMLV) and xenotropic MuLV-related virus (XMRV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), togaviridae: sindbis virus (SINV) and Ross river virus (RRV). Specifically targets the multiply spliced but not unspliced or singly spliced HIV-1 mRNAs for degradation. Isoform 1 is a more potent viral inhibitor than isoform 2. Isoform 2 acts as a positive regulator of DDX58/RIG-I signaling resulting in activation of the downstream effector IRF3 leading to the expression of type I IFNs and IFN stimulated genes (ISGs). 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; RNA-binding

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: Golgi apparatus; cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; NAD+ ADP-ribosyltransferase activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of viral genome replication; metabolic process; response to virus; positive regulation of interferon-beta production; innate immune response; defense response to virus; positive regulation of interferon-alpha production