Rabbit anti-Human ADAMTS13 Polyclonal Antibody | anti-ADAMTS13 antibody

ADAMTS13, ID (von Willebrand Factor-cleaving Protease, vWF-cleaving Protease, vWF-CP, A Disintegrin And Metalloproteinase With Thrombospondin Motifs 13, ADAMTS-13, ADAM-TS 13, ADAM-TS13, UNQ6102/PRO20085, C9orf8) (MaxLight 490)

Cat. Num. AAA6461516

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ADAMTS13; Polyclonal Antibody; ID (von Willebrand Factor-cleaving Protease; vWF-cleaving Protease; vWF-CP; A Disintegrin And Metalloproteinase With Thrombospondin Motifs 13; ADAMTS-13; ADAM-TS 13; ADAM-TS13; UNQ6102/PRO20085; C9orf8) (MaxLight 490); anti-ADAMTS13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ADAMTS13.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
• Sequence Length: 1427

• Applicable Applications for anti-ADAMTS13 antibody: FLISA, Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 829-858 in the central region of human ADAMTS13.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS630150. ADAMTS13 (arrow) was detected usingMBS630150.)

Immunohistochemistry (IHC)

(Immunohistochemistry in formalin-fixed and paraffin-embedded human hepatocarcinoma usingMBS630150, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Related Product Information for anti-ADAMTS13 antibody

ADAMTS13 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme is the von Willebrand Factor (vWF)-cleaving protease, which is responsible for cleaving at the site of Tyr842-Met843 of the vWF molecule. A deficiency of this enzyme is associated with thrombotic thrombocytopenic purpura.

Product Categories/Family for anti-ADAMTS13 antibody

Antibodies; Matrix Metalloproteinases

References

1. Zheng X., Chung D., Takayama T.K.J. Biol. Chem. 276:41059-41063(2001). 2. Levy G.G., Nichols W.C.Nature 413:488-494(2001). 3. Cal S., Obaya A.J., Llamazares M., Garabaya C.Gene 283:49-62(2002). 4. Zheng X., Nishio K., Majerus E.M.J. Biol. Chem. 278:30136-30141(2003). 5. Anderson P.J., Kokame K., Sadler J.E.J. Biol. Chem. 281:850-857(2006). 6. Pimanda J.E., Maekawa A., Wind T.Blood 103:627-629(2004). 7. Plaimauer B., Fuhrmann J., Mohr G.Blood 107:118-125(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 21265034
• NCBI Accession #: NP_620594
• NCBI GenBank Nucleotide #: NP_620594.1
• UniProt Accession #: Q76LX8
• Molecular Weight: 153.604
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 13 isoform 1 preproprotein
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 13
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: ADAMTS13
• UniProt Synonym Gene Names: C9orf8; ADAM-TS 13; ADAM-TS13; ADAMTS-13; vWF-CP; vWF-cleaving protease

Uniprot Description

Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation.