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Western Blot (WB)
(Western Blot (WB) analysis of ZC3H8 pAb at 1:500 dilutionLane1:HCT116 whole cell lysate (20ug) Lane2:Hela whole cell lysate (20ug) Lane3:C6 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug))
Rabbit ZC3H8 Polyclonal Antibody | anti-ZC3H8 antibody
ZC3H8 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
ZC3H8; Polyclonal Antibody; ZC3H8 Polyclonal Antibody; Zinc finger CCCH domain-containing protein 8; ZC3HDC8; anti-ZC3H8 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ZC3H8 polyclonal antibody detects endogenous levels of ZC3H8 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-ZC3H8 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 133-178 of Human ZC3H8.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of ZC3H8 pAb at 1:500 dilutionLane1:HCT116 whole cell lysate (20ug) Lane2:Hela whole cell lysate (20ug) Lane3:C6 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of ZC3H8 pAb at 1:500 dilutionLane1:HCT116 whole cell lysate (20ug) Lane2:Hela whole cell lysate (20ug) Lane3:C6 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug))
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of ZC3H8 pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of ZC3H8 pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of ZC3H8 pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of ZC3H8 pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of ZC3H8 pAb in paraffin-embedded human rectum carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of ZC3H8 pAb in paraffin-embedded human rectum carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)
Related Product Information for anti-ZC3H8 antibody
Zinc-finger CCCH domain-containing protein 8 (ZC3H8) acts as a transcriptional repressor of the GATA3 promoter. It is a sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (by similarity). ZC3H8 induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
~ 33kDa
Protein Family
Similar Products
Product Notes
The ZC3H8 (Catalog #AAA3005937) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZC3H8 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ZC3H8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the ZC3H8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZC3H8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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