Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using ZC3H7B antibody.)
Rabbit anti-Human, Mouse ZC3H7B Polyclonal Antibody | anti-ZC3H7B antibody
ZC3H7B Antibody
Gene Names
ZC3H7B; RoXaN
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ZC3H7B; Polyclonal Antibody; ZC3H7B Antibody; DKFZp434K0920; FLJ13787; KIAA1031; Rotavirus 'X' associated non structural protein; Rotavirus X protein associated with NSP3; RoXaN; Ubiquitous tetratricopeptide containing protein RoXaN; Zinc finger CCCH domain containing protein 7B; Zinc finger CCCH type containing 7B; anti-ZC3H7B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ZC3H7B antibody detects endogenous levels of total ZC3H7B
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
977
Applicable Applications for anti-ZC3H7B antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Nuclear
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%), Xenopus (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using ZC3H7B antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using ZC3H7B antibody.)
Immunofluorescene (IF)
(MBS9604924 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9604924 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using ZC3H7B Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using ZC3H7B Antibody.)
Related Product Information for anti-ZC3H7B antibody
Function: May be a specific regulator of miRNA biogenesis. Binds to microRNAs MIR7-1, MIR16-2 and MIR29A hairpins recognizing the 'ATA(A/T)' motif in the apical loop.
Subunit Structure: Interacts (via LD motif) with rotavirus A NSP3 (via the coiled-coil region).
Subunit Structure: Interacts (via LD motif) with rotavirus A NSP3 (via the coiled-coil region).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 111 kD
Predicted: 112 kDa
Predicted: 112 kDa
NCBI Official Full Name
zinc finger CCCH domain-containing protein 7B
NCBI Official Synonym Full Names
zinc finger CCCH-type containing 7B
NCBI Official Symbol
ZC3H7B
NCBI Official Synonym Symbols
RoXaN
NCBI Protein Information
zinc finger CCCH domain-containing protein 7B
UniProt Protein Name
Zinc finger CCCH domain-containing protein 7B
Protein Family
UniProt Gene Name
ZC3H7B
UniProt Synonym Gene Names
KIAA1031; RoXaN
NCBI Description
This gene encodes a protein that contains a tetratricopeptide repeat domain. The encoded protein also interacts with the rotavirus non-structural protein NSP3. [provided by RefSeq, Jul 2008]
Uniprot Description
May be a specific regulator of miRNA biogenesis. Binds to microRNAs MIR7-1, MIR16-2 and MIR29A hairpins recognizing the 'ATA(A/T)' motif in the apical loop.
Research Articles on ZC3H7B
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Product Notes
The ZC3H7B zc3h7b (Catalog #AAA9604924) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZC3H7B Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ZC3H7B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the ZC3H7B zc3h7b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZC3H7B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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