Western Blot (WB)
(Western blot analysis of ZBTB25 expression in HeLa (A), mouse brain (B), mouse heart (C) whole cell lysates.)
Rabbit anti-Human, Mouse ZBTB25 Polyclonal Antibody | anti-ZBTB25 antibody
Anti-ZBTB25 Antibody
Gene Names
ZBTB25; KUP; ZNF46; C14orf51
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Purified by Immunogen Affinity Chromatography
Synonyms
ZBTB25; Polyclonal Antibody; Anti-ZBTB25 Antibody; C14orf51; KUP; ZNF46; Zinc finger and BTB domain-containing protein 25; Zinc finger protein 46; Zinc finger protein KUP; anti-ZBTB25 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of ZBTB25 protein.
Purity/Purification
Purified by Immunogen Affinity Chromatography
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Applicable Applications for anti-ZBTB25 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1:500-1:2000
IF/ICC: 1:50-1:200
IF/ICC: 1:50-1:200
Immunogen
Recombinant fusion protein of human ZBTB25. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of ZBTB25 expression in HeLa (A), mouse brain (B), mouse heart (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of ZBTB25 expression in HeLa (A), mouse brain (B), mouse heart (C) whole cell lysates.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of ZBTB25 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of ZBTB25 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Related Product Information for anti-ZBTB25 antibody
Rabbit polyclonal antibody to ZBTB25
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
435
NCBI Official Full Name
Zinc finger and BTB domain-containing protein 25
NCBI Official Synonym Full Names
zinc finger and BTB domain containing 25
NCBI Official Symbol
ZBTB25
NCBI Official Synonym Symbols
KUP; ZNF46; C14orf51
NCBI Protein Information
zinc finger and BTB domain-containing protein 25; zinc finger protein 46; zinc finger protein KUP
UniProt Protein Name
Zinc finger and BTB domain-containing protein 25
Protein Family
UniProt Gene Name
ZBTB25
UniProt Synonym Gene Names
C14orf51; KUP; ZNF46
UniProt Entry Name
ZBT25_HUMAN
Uniprot Description
ZBTB25: May be involved in transcriptional regulation.
Protein type: C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 14q23-q24
Cellular Component: nucleoplasm; cytoplasm
Molecular Function: protein binding; DNA binding; metal ion binding; transcription factor activity
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; gene expression
Research Articles on ZBTB25
Similar Products
Product Notes
The ZBTB25 zbtb25 (Catalog #AAA8308665) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ZBTB25 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ZBTB25 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:2000 IF/ICC: 1:50-1:200. Researchers should empirically determine the suitability of the ZBTB25 zbtb25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZBTB25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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