Western Blot (WB)
(Western blot analysis of ZBTB16 expression in HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate, The lane on the left is treated with the antigen-specific peptide.)
Rabbit ZBTB16 Polyclonal Antibody | anti-ZBTB16 antibody
ZBTB16 Antibody
Gene Names
ZBTB16; PLZF; ZNF145
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ZBTB16; Polyclonal Antibody; ZBTB16 Antibody; AI467657; lu; PLZF; Promyelocytic leukemia zinc finger protein; ZBT16_HUMAN; Zfp145; Zinc finger and BTB domain containing 16; zinc finger and BTB domain containing protein 16; Zinc finger and BTB domain-containing protein 16; Zinc finger protein 145 (Kruppel like expressed in promyelocytic leukemia); Zinc finger protein 145; Zinc finger protein PLZF; Znf145; anti-ZBTB16 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ZBTB16 antibody detects endogenous levels of total ZBTB16
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
673
Applicable Applications for anti-ZBTB16 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide
Subcellular Location
Nucleus.
Tissue Specificity
Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung.
Predicted Cross Reactivity
Pig, Bovine, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Rabbit (92%), Dog (92%), Chicken (82%)
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of ZBTB16 expression in HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of ZBTB16 expression in HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-ZBTB16 antibody
Description: Probable transcription factor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Function: Acts as a transcriptional repressor (PubMed:24359566). May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:14528312).
Subunit Structure: Binds EPN1 (PubMed:11161217). Interacts with ZBTB32 and CUL3 (PubMed:10572087, PubMed:14528312). Interacts with ATP7B (PubMed:16676348). Interacts (via C2H2-type zinc finger domains 1 and 2) with RNF112 (PubMed:24359566).
Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family.
Function: Acts as a transcriptional repressor (PubMed:24359566). May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:14528312).
Subunit Structure: Binds EPN1 (PubMed:11161217). Interacts with ZBTB32 and CUL3 (PubMed:10572087, PubMed:14528312). Interacts with ATP7B (PubMed:16676348). Interacts (via C2H2-type zinc finger domains 1 and 2) with RNF112 (PubMed:24359566).
Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 85 kDa
Predicted: 75 kDa
Predicted: 75 kDa
NCBI Official Full Name
zinc finger and BTB domain-containing protein 16 isoform 1
NCBI Official Synonym Full Names
zinc finger and BTB domain containing 16
NCBI Official Symbol
ZBTB16
NCBI Official Synonym Symbols
PLZF; ZNF145
NCBI Protein Information
zinc finger and BTB domain-containing protein 16
UniProt Protein Name
Zinc finger and BTB domain-containing protein 16
Protein Family
UniProt Gene Name
ZBTB16
UniProt Synonym Gene Names
PLZF; ZNF145
NCBI Description
This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
Acts as a transcriptional repressor (PubMed:24359566). May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:14528312).
Research Articles on ZBTB16
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Product Notes
The ZBTB16 zbtb16 (Catalog #AAA9604125) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZBTB16 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ZBTB16 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ZBTB16 zbtb16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZBTB16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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