Rabbit anti-Human, Mouse ZAP70 Polyclonal Antibody | anti-ZAP70 antibody

ZAP70 (70kD zeta-chain Associated Protein, ZAP-70, Tyrosine-protein Kinase ZAP-70, Syk-related Tyrosine Kinase, SRK, STCD, STD, TZK) (PE)

Cat. Num. AAA6398897

• Gene Names: ZAP70; SRK; STD; TZK; STCD; ZAP-70
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ZAP70; Polyclonal Antibody; ZAP70 (70kD zeta-chain Associated Protein; ZAP-70; Tyrosine-protein Kinase ZAP-70; Syk-related Tyrosine Kinase; SRK; STCD; STD; TZK) (PE); Zeta-chain (TCR) Associated Protein Kinase 70kDa; EC=2.7.10.2; anti-ZAP70 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ZAP70. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ZAP70 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human ZAP70, aa1-619 (AAH53878.1).
• Immunogen Sequence: MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRKPCNRPSGLEPQPGVFDCLRDAMVRDYVRQTWKLEGEALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGAQTDGKFLLRPRKEQGTYALSLIYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYCLKEACPNSSASNASGAAAPTLPAHPSTLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALMSDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ZAP70 rabbit polyclonal antibody. Western Blot analysis of ZAP70 expression in mouse spleen.)

Western Blot (WB)

(Western Blot analysis of ZAP70 expression in transfected 293T cell line by ZAP70 polyclonal antibody. Lane 1: ZAP70 transfected lysate (69.9kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-ZAP70 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 31753147
• NCBI GeneID: 7535
• NCBI Accession #: AAH53878.1
• NCBI GenBank Nucleotide #: NM_001079
• Molecular Weight: Calculated MW: 70; Observed MW: 70
• NCBI Official Full Name: Zeta-chain (TCR) associated protein kinase 70kDa
• NCBI Official Synonym Full Names: zeta chain of T cell receptor associated protein kinase 70kDa
• NCBI Official Symbol: ZAP70
• NCBI Official Synonym Symbols: SRK; STD; TZK; STCD; ZAP-70
• NCBI Protein Information: tyrosine-protein kinase ZAP-70
• Protein Family: Tyrosine-protein kinase

NCBI Description

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]