Rat Protein phosphatase 1 regulatory subunit 1B (PPP1R1B) ELISA Kit | PPP1R1B elisa kit

Rat Protein phosphatase 1 regulatory subunit 1B (PPP1R1B) ELISA Kit

• Reactivity: Rat
• Synonyms: Protein phosphatase 1 regulatory subunit 1B (PPP1R1B); Rat Protein phosphatase 1 regulatory subunit 1B (PPP1R1B) ELISA Kit; PPP1R1B elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of PPP1R1B. No significant cross-reactivity or interference between PPP1R1B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PPP1R1B and all the analogues, therefore, cross reaction may still exist in some cases.

• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for PPP1R1B elisa kit

Principle of the Assay: PPP1R1B ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PPP1R1B antibody and an PPP1R1B-HRP conjugate. The assay sample and buffer are incubated together with PPP1R1B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PPP1R1B concentration since PPP1R1B from samples and PPP1R1B-HRP conjugate compete for the anti-PPP1R1B antibody binding site. Since the number of sites is limited, as more sites are occupied by PPP1R1B from the sample, fewer sites are left to bind PPP1R1B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PPP1R1B concentration in each sample is interpolated from this standard curve.

Intended Uses: This PPP1R1B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PPP1R1B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for PPP1R1B elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 27807175
• NCBI GeneID: 282459
• NCBI Accession #: NP_777072.1
• NCBI GenBank Nucleotide #: NM_174647.2
• Molecular Weight: 22,614 Da
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 1B
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory (inhibitor) subunit 1B<
• NCBI Official Symbol: PPP1R1B
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 1B; DARPP-32; dopamine and cAMP regulated; dopamine- and cAMP-regulated neuronal phosphoprotein; protein phosphatase 1, regulatory (inhibitor) subunit 1B (dopamine and cAMP regulated phosphoprotein, DARPP-32)
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 1B
• UniProt Gene Name: PPP1R1B
• UniProt Entry Name: PPR1B_BOVIN