Rat amyloid beta peptide 1-42 ELISA Kit | Abeta1-42 elisa kit

Rat amyloid beta peptide 1-42 ELISA Kit

• Gene Names: App; Ag; Abpp; Adap; Cvap; Abeta; betaApp; E030013M08Rik
• Reactivity: Rat
• Synonyms: amyloid beta peptide 1-42; Rat amyloid beta peptide 1-42 ELISA Kit; amyloid beta peptide 1-42 (Rat); Abeta1-42 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Abeta1~42. No significant cross-reactivity or interference between Abeta1~42 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Abeta1~42 and all the analogues, therefore, cross reaction may still exist in some cases.

• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for Abeta1-42 elisa kit

Principle of the Assay: Abeta1~42 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-Abeta1~42 antibody and an Abeta1~42-HRP conjugate. The assay sample and buffer are incubated together with Abeta1~42-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Abeta1~42 concentration since Abeta1~42 from samples and Abeta1~42-HRP conjugate compete for the anti-Abeta1~42 antibody binding site. Since the number of sites is limited, as more sites are occupied by Abeta1~42 from the sample, fewer sites are left to bind Abeta1~42-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Abeta1~42 concentration in each sample is interpolated from this standard curve.

Intended Uses: This Abeta1~42 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat Abeta1~42. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for Abeta1-42 elisa kit

Rat ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 311893408
• NCBI GeneID: 11820
• NCBI Accession #: NP_001185755.1
• NCBI GenBank Nucleotide #: NM_001198826.1
• UniProt Accession #: P12023; P97487; P97942; Q99K32
• Molecular Weight: 86,722 Da
• NCBI Official Full Name: amyloid beta A4 protein isoform 6
• NCBI Official Synonym Full Names: amyloid beta (A4) precursor protein
• NCBI Official Symbol: App
• NCBI Official Synonym Symbols: Ag; Abpp; Adap; Cvap; Abeta; betaApp; E030013M08Rik
• NCBI Protein Information: amyloid beta A4 protein; appican; protease nexin II; amyloidogenic glycoprotein; alzheimer disease amyloid A4 protein homolog
• UniProt Protein Name: Amyloid beta A4 protein
• UniProt Gene Name: App
• UniProt Synonym Gene Names: APP; AG; S-APP-alpha; S-APP-beta; AID(59); AID(57); AID(50)
• UniProt Entry Name: A4_MOUSE