Rabbit anti-Human ZAP70 Polyclonal Antibody | anti-ZAP70 antibody

ZAP70, NT (ZAP70, SRK, Tyrosine-protein kinase ZAP-70, 70kD zeta-chain associated protein, Syk-related tyrosine kinase) (FITC)

Cat. Num. AAA6364482

• Gene Names: ZAP70; SRK; STD; TZK; ZAP-70; FLJ17670; FLJ17679; ZAP70
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ZAP70; Polyclonal Antibody; NT (ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70kD zeta-chain associated protein; Syk-related tyrosine kinase) (FITC); Syk-related tyrosine kinase; anti-ZAP70 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ZAP70 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-130 amino acids from the N-terminal region of human ZAP70.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ZAP70 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ZAP70 antibody detected the ZAP70 protein (arrow).)

Immunohistochemistry (IHC)

(ZAP70 Antibody (N-term) (AP14146a)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZAP70 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-ZAP70 antibody

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Product Categories/Family for anti-ZAP70 antibody

Antibodies; Protein Kinases

References

Zanotti, R., et al. Am. J. Hematol. 85(7):494-498(2010). Lin, Y.P., et al. Mol. Immunol. 47 (11-12), 2022-2029 (2010): Liu, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(22):10166-10171(2010). Kotaskova, J., et al. J Mol Diagn 12(3):328-334(2010). Trojani, A., et al. Cancer Biomark 6(1):1-9(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 46488944
• NCBI GeneID: 7535
• NCBI Accession #: NP_997402.1; NP_001070.2
• NCBI GenBank Nucleotide #: NM_207519.1
• UniProt Accession #: P43403; Q6PIA4; Q8IXD6; Q9UBS6
• Molecular Weight: 35516 Da
• NCBI Official Full Name: zeta-chain associated protein kinase 70kDa isoform 2 [Homo sapiens]
• NCBI Official Synonym Full Names: zeta-chain (TCR) associated protein kinase 70kDa
• NCBI Official Symbol: ZAP70
• NCBI Official Synonym Symbols: SRK; STD; TZK; ZAP-70; FLJ17670; FLJ17679; ZAP70
• NCBI Protein Information: zeta-chain associated protein kinase 70kDa
• UniProt Protein Name: Tyrosine-protein kinase ZAP-70
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ZAP70
• UniProt Synonym Gene Names: SRK
• UniProt Entry Name: ZAP70_HUMAN

Uniprot Description

ZAP70: a tyrosine kinase of the Syk family. Associates with the T-cell antigen receptor zeta-chain after TCR stimulation. Phosphorylated by Src-family kinases following antigen receptor activation. Plays a role in lymphocyte activation.

Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; EC 2.7.10.2; TK group; Syk family

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; immunological synapse; intercellular junction; cytosol; lipid raft

Molecular Function: protein binding; phosphotyrosine binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: cell migration; adaptive immune response; peptidyl-tyrosine phosphorylation; B cell activation; T cell activation; positive regulation of calcium-mediated signaling; negative thymic T cell selection; protein amino acid phosphorylation; T cell receptor signaling pathway; positive regulation of T cell differentiation; innate immune response; immune response; positive regulation of alpha-beta T cell proliferation; beta selection; positive regulation of alpha-beta T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; positive thymic T cell selection

Disease: Selective T-cell Defect