Rabbit anti-Human, Mouse Z385B Polyclonal Antibody | anti-Z385B antibody

Z385B, NT (ZNF385B, ZNF533, Zinc finger protein 385B, Zinc finger protein 533) (APC)

Cat. Num. AAA6364436

• Gene Names: ZNF385B; ZNF533
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Z385B; Polyclonal Antibody; NT (ZNF385B; ZNF533; Zinc finger protein 385B; Zinc finger protein 533) (APC); ZNF385B; Zinc finger protein 533; anti-Z385B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Z385B antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Z385B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Z385B.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Z385B Antibody (N-term) western blot analysis in mouse liver tissue lysates (15ug/lane).This demonstrates the Z385B antibody detected the Z385B protein (arrow).)

Immunohistochemistry (IHC)

(Z385B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Z385B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Z385B antibody

ZNF385B contains 4 U1-type zinc fingers. ZNF385B is expressed in brain with a potential role in RNA maturation, stability, or transcription regulation. There are 5 known isoforms.

Product Categories/Family for anti-Z385B antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Maestrini, E., et al. Mol. Psychiatry (2009). In press: Trynka, G., et al. Gut (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 63029928
• NCBI GeneID: 151126
• NCBI Accession #: NP_689733.3
• NCBI GenBank Nucleotide #: NM_152520.4
• UniProt Accession #: Q569K4; Q49A04; Q6ZMZ7; Q8IY01; Q8N8H2; Q96DK4
• Molecular Weight: 45,476 Da
• NCBI Official Full Name: zinc finger protein 385B isoform 1
• NCBI Official Synonym Full Names: zinc finger protein 385B
• NCBI Official Symbol: ZNF385B
• NCBI Official Synonym Symbols: ZNF533
• NCBI Protein Information: zinc finger protein 385B
• UniProt Protein Name: Zinc finger protein 385B
• UniProt Gene Name: ZNF385B
• UniProt Synonym Gene Names: ZNF533
• UniProt Entry Name: Z385B_HUMAN

Uniprot Description

Function: May play a role in p53/TP53-mediated apoptosis. Ref.3

Subunit structure: Interacts with p53/TP53; the interaction is direct. Ref.3

Subcellular location: Nucleus Ref.3.

Tissue specificity: Detected in germinal center of lymph node (at protein level). Expressed in spleen, lymph node and tonsil. Ref.3

Sequence similarities: Contains 4 matrin-type zinc fingers.