Western Blot (WB)
(Western blot analysis of total cell lysate (200 mug) from serum stimulated bovine endothelial cells (lane 1) and Hela cells (lane 2) probed With chicken anti-human VY1 antibody. lane 2 shows that the antibody detects O-glycosylated, unmodified and calpain cleavage product.)
Chicken YY1 Polyclonal Antibody | anti-YY1 antibody
YY1 Chicken Polyclonal Antibody
Gene Names
yy1.S; yy1; xyy1; nf-e1; ucrbp; yy1-a; yy1-b; ino80s; yin-yang-1
Reactivity
Human, Pig, Sheep, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Synonyms
YY1; Polyclonal Antibody; YY1 Chicken Polyclonal Antibody; anti-YY1 antibody
Host
Chicken
Reactivity
Human, Pig, Sheep, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody recognizes the Transcription Factor YY1; in WB, the antibody recognizes the O-glycosylated, the phosphorylated, and the post-translationally unmodified protein in cellular and tissue lysates.
Form/Format
Lyophilized purified total IgY in PBS pH7.4
Sequence Length
370
Applicable Applications for anti-YY1 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
If reconstituted in 30 muL: WB: 1:3000-1:8000; IF: 1:500-1:1000 (in tissue paraffin sections)
Immunogen
Recombinant human YY1
Preservative
Ampicillin and streptomycin, 0.05% sodium aside.
Preparation and Storage
Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage.
To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).
For maximum recovery of product centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).
For maximum recovery of product centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of total cell lysate (200 mug) from serum stimulated bovine endothelial cells (lane 1) and Hela cells (lane 2) probed With chicken anti-human VY1 antibody. lane 2 shows that the antibody detects O-glycosylated, unmodified and calpain cleavage product.)
Western Blot (WB)
(Western blot analysis of total cell lysate (200 mug) from serum stimulated bovine endothelial cells (lane 1) and Hela cells (lane 2) probed With chicken anti-human VY1 antibody. lane 2 shows that the antibody detects O-glycosylated, unmodified and calpain cleavage product.)
Immunofluorescence (IF)
(Immunofluorescence staining of pig iliac artery cryo sections. YY1 fIuorescent immunostalning is shown in red. The nuclear DAPl counter stain is shown in blue Control stainng WIth pre-immune chicken IgY is shown on the right Magnification: 20)
Immunofluorescence (IF)
(Immunofluorescence staining of pig iliac artery cryo sections. YY1 fIuorescent immunostalning is shown in red. The nuclear DAPl counter stain is shown in blue Control stainng WIth pre-immune chicken IgY is shown on the right Magnification: 20)
Related Product Information for anti-YY1 antibody
YY1 is a ubiquitous and multifunctional zinc-finger transcription factor involved in the activation or repression of a large number of genes that are critical for basic biological processes of cell growth, development, differentiation, cell cycle and even programmed cell death (apoptosis). YY1 is an essential protein; deletion of YY1 leads to lethality in mouse embryos and disruption of one allele causing developmental defects.
References
1. Lange M-Arterial territory-specific phosphorylated retinoblastoma protein species and CDK2 promote differences in the vascular smooth muscle cell response…
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
YY1 transcription factor S homeolog
NCBI Official Synonym Full Names
YY1 transcription factor S homeolog
NCBI Official Symbol
yy1.S
NCBI Official Synonym Symbols
yy1; xyy1; nf-e1; ucrbp; yy1-a; yy1-b; ino80s; yin-yang-1
NCBI Protein Information
YY1 transcription factor S homeolog
Protein Family
Similar Products
Product Notes
The YY1 (Catalog #AAA588244) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The YY1 Chicken Polyclonal Antibody reacts with Human, Pig, Sheep, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's YY1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). If reconstituted in 30 muL: WB: 1:3000-1:8000; IF: 1:500-1:1000 (in tissue paraffin sections). Researchers should empirically determine the suitability of the YY1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YY1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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