Immunohistochemistry (IHC)
(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit YWHAH/14-3-3 Eta Polyclonal Antibody | anti-YWHAH antibody
Anti-YWHAH/14-3-3 Eta Antibody (Internal)
Predicted: Mouse, Bovine, Rat, Monkey
Predicted: Mouse, Bovine, Rat, Monkey
IHC-P: 1:50
WB: 1:500-1:1000
Aliquot to avoid freeze/thaw cycles.
Immunohistochemistry (IHC)
(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunofluorescence (IF)
(Immunofluorescent analysis of 14-3-3 eta staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of 14-3-3 eta staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Western Blot (WB)
(Western blot analysis of 14-3-3 eta expression in MCF7 (A); SP2/0 (B); H9C2 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of 14-3-3 eta expression in MCF7 (A); SP2/0 (B); H9C2 (C) whole cell lysates.)
NCBI and Uniprot Product Information
NCBI Description
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009]
Uniprot Description
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
Research Articles on YWHAH
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Product Notes
The YWHAH ywhah (Catalog #AAA2401421) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-YWHAH/14-3-3 Eta Antibody (Internal) reacts with Human Predicted: Mouse, Bovine, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's YWHAH/14-3-3 Eta can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ICC: 1:100-1:500 IHC-P: 1:50 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the YWHAH ywhah for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YWHAH/14-3-3 Eta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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