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Western Blot (WB) (Western blot analysis of extracts from Jurkat cells, using 14-3-3 eta antibody.)

Rabbit 14-3-3 eta Polyclonal Antibody | anti-14-3-3 eta antibody

14-3-3 eta Antibody

Gene Names
YWHAH; YWHA1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
14-3-3 eta; Polyclonal Antibody; 14-3-3 eta Antibody; 14-3-3 protein eta; Protein AS1; YWHAH; YWHA1; anti-14-3-3 eta antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total 14-3-3 eta protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
246
Applicable Applications for anti-14-3-3 eta antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from internal of human 14-3-3 eta.
Target Name
14-3-3 eta
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells, using 14-3-3 eta antibody.)

Western Blot (WB) (Western blot analysis of extracts from Jurkat cells, using 14-3-3 eta antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells, using 14-3-3 eta antibody.)

Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells, using 14-3-3 eta antibody.)
Related Product Information for anti-14-3-3 eta antibody
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.Swanson K.D., Biochim. Biophys. Acta 1216:145-148(1993).Ichimura-Ohshima Y., J. Neurosci. Res. 31:600-605(1992).Muratake T., Mol. Neurobiol. 11:223-230(1995).
Product Categories/Family for anti-14-3-3 eta antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,219 Da
NCBI Official Full Name
14-3-3 protein eta
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
NCBI Official Symbol
YWHAH
NCBI Official Synonym Symbols
YWHA1
NCBI Protein Information
14-3-3 protein eta
UniProt Protein Name
14-3-3 protein eta
UniProt Gene Name
YWHAH
UniProt Synonym Gene Names
YWHA1
UniProt Entry Name
1433F_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009]

Uniprot Description

14-3-3 eta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Nuclear receptor co-regulator; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: cytoplasmic vesicle membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: protein domain specific binding; insulin-like growth factor receptor binding; protein binding; enzyme binding; sodium channel regulator activity; protein heterodimerization activity; actin binding; glucocorticoid receptor binding

Biological Process: intracellular protein transport; substantia nigra development; regulation of neuron differentiation; apoptosis; positive regulation of transcription, DNA-dependent; glucocorticoid receptor signaling pathway; glucocorticoid catabolic process; regulation of synaptic plasticity; negative regulation of dendrite morphogenesis; regulation of sodium ion transport

Research Articles on 14-3-3 eta

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Product Notes

The 14-3-3 eta ywhah (Catalog #AAA9402131) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The 14-3-3 eta Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's 14-3-3 eta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Western blotting: 1:500~1:3000 Immunofluorescence: 1:100~1:500. Researchers should empirically determine the suitability of the 14-3-3 eta ywhah for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3 eta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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