Rabbit YWHAG Polyclonal Antibody | anti-YWHAG antibody

YWHAG Antibody

Cat. Num. AAA9410455

• Gene Names: YWHAG; PPP1R170; 14-3-3GAMMA
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Synonyms: YWHAG; Polyclonal Antibody; YWHAG Antibody; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; gamma polypeptide; 14-3-3GAMMA; anti-YWHAG antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total YWHAG protein.
• Form/Format: Supplied at 3.6mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
• Sequence Length: 247

• Applicable Applications for anti-YWHAG antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:2000-1:10000; Western blotting: 1:1000-1:5000; Immunohistochemistry: 1:50-1:200
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to a region derived from 141-154 amino acids of Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
• Target Name: YWHAG
• Preparation and Storage: Store at -20 degree C/1 year

Testing Data

(Gel: 10%SDS-PAGELane1: Hela cell lysateLane2: 293T cell lysateLane3: Jurkat cell lysateLysates: 40 ug per lanePrimary antibody: 1/1800 dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 10 seconds)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 31246 (YWHAG Antibody) at dilution 1/100, on the right is treated with the synthetic peptide.)

Related Product Information for anti-YWHAG antibody

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

Product Categories/Family for anti-YWHAG antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 21464101
• NCBI GeneID: 7532
• NCBI Accession #: NP_036611
• NCBI GenBank Nucleotide #: NM_012479.3
• Molecular Weight: 28,303 Da
• NCBI Official Full Name: 14-3-3 protein gamma
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma
• NCBI Official Symbol: YWHAG
• NCBI Official Synonym Symbols: PPP1R170; 14-3-3GAMMA
• NCBI Protein Information: 14-3-3 protein gamma
• UniProt Protein Name: 14-3-3 protein gamma
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAG
• UniProt Synonym Gene Names: KCIP-1
• UniProt Entry Name: 1433G_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]

Uniprot Description

14-3-3 gamma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Homodimer. Interacts with SAMSN1. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT. Highly expressed in brain, skeletal muscle, and heart. Belongs to the 14-3-3 family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: cytoplasmic vesicle membrane; focal adhesion; membrane; cytosol

Molecular Function: protein kinase C inhibitor activity; insulin-like growth factor receptor binding; protein domain specific binding; protein binding; protein kinase C binding; receptor tyrosine kinase binding

Biological Process: cellular response to insulin stimulus; regulation of neuron differentiation; apoptosis; organelle organization and biogenesis; regulation of synaptic plasticity; regulation of signal transduction; negative regulation of protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein targeting