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Immunohistochemistry (IHC)
(YI016 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of YI016 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human YI016 Polyclonal Antibody | anti-YI016 antibody
YI016, CT (Putative tubulin beta chain-like protein ENSP00000290377) (Biotin)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
YI016; Polyclonal Antibody; CT (Putative tubulin beta chain-like protein ENSP00000290377) (Biotin); Putative tubulin beta chain-like protein ENSP00000290377; anti-YI016 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-YI016 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
IF: 1:10-50
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
IF: 1:10-50
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
YI016 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-288 amino acids from the C-terminal region of human YI016.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(YI016 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of YI016 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(YI016 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of YI016 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Western Blot (WB)
(YI016 Antibody (C-term) western blot analysis in K562,HL-60,NCI-H460 cell line lysates (35ug/lane).This demonstrates the YI016 antibody detected the YI016 protein (arrow).)
Western Blot (WB)
(YI016 Antibody (C-term) western blot analysis in K562,HL-60,NCI-H460 cell line lysates (35ug/lane).This demonstrates the YI016 antibody detected the YI016 protein (arrow).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of YI016 Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of YI016 Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC/FACS)
(YI016 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(YI016 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-YI016 antibody
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity).
Product Categories/Family for anti-YI016 antibody
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Product Notes
The YI016 (Catalog #AAA6364261) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The YI016, CT (Putative tubulin beta chain-like protein ENSP00000290377) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's YI016 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 IF: 1:10-50 IHC: 1:10-50 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the YI016 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YI016, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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