Western Blot (WB)
(Western blot analysis in K562 cell line lysates (35ug/lane) usingMBS6008022. This demonstrates MBS6008022 detected the YBX1 protein (arrow).)
Rabbit anti-Human YBX1 Polyclonal Antibody | anti-YBX1 antibody
YBX1, ID (YBX1, NSEP1, YB1, Nuclease-sensitive element-binding protein 1, CCAAT-binding transcription factor I subunit A, DNA-binding protein B, Enhancer factor I subunit A, Y-box transcription factor, Y-box-binding protein 1) (MaxLight 405)
Western Blot (WB)
(Western blot analysis in K562 cell line lysates (35ug/lane) usingMBS6008022. This demonstrates MBS6008022 detected the YBX1 protein (arrow).)
Western Blot (WB)
(Western blot analysis in K562 cell line lysates (35ug/lane) usingMBS6008022. This demonstrates MBS6008022 detected the YBX1 protein (arrow).)
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained withMBS6008022. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with YBX1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). YBX1 immunoreactivity is localized to Cytoplasm significantly.)
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained withMBS6008022. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with YBX1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). YBX1 immunoreactivity is localized to Cytoplasm significantly.)
NCBI and Uniprot Product Information
Uniprot Description
YB-1: a nuclear protein that binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors CCAAT-containing Y-box of HLA class II genes. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP. Involved in cisplatin resistance. Seems to be a negative regulatory factor. May play a role in both transcriptional and translational regulation of spermatogenesis. Found at very low levels at day 10 and levels increase at day 15 and persist throughout adulthood.
Protein type: Transcription factor; RNA-binding; RNA splicing; RNA processing
Chromosomal Location of Human Ortholog: 1p34
Cellular Component: nucleoplasm; nuclear membrane; intracellular membrane-bound organelle; stress granule; cytoplasm; nucleus; ribonucleoprotein complex; U12-dependent spliceosome
Molecular Function: protein binding; DNA binding; RNA binding; double-stranded DNA binding; transcription factor activity; single-stranded DNA binding
Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; negative regulation of striated muscle cell differentiation; regulation of transcription, DNA-dependent; in utero embryonic development; positive regulation of cell division; RNA splicing; positive regulation of transcription from RNA polymerase II promoter; gene expression; negative regulation of transcription from RNA polymerase II promoter
Research Articles on YBX1
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Product Notes
The YBX1 ybx1 (Catalog #AAA6364198) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The YBX1, ID (YBX1, NSEP1, YB1, Nuclease-sensitive element-binding protein 1, CCAAT-binding transcription factor I subunit A, DNA-binding protein B, Enhancer factor I subunit A, Y-box transcription factor, Y-box-binding protein 1) (MaxLight 405) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's YBX1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), FLISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the YBX1 ybx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YBX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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