Western Blot (WB)
(Western Blot (WB) analysis of YAF2 polyclonal antibody at 1:500 dilutionLane1:Jurkat whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)
Rabbit YAF2 Polyclonal Antibody | anti-YAF2 antibody
YAF2 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
YAF2; Polyclonal Antibody; YAF2 Polyclonal Antibody; YY1-associated factor 2; anti-YAF2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
YAF2 polyclonal antibody detects endogenous levels of YAF2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
138
Applicable Applications for anti-YAF2 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human YAF2.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of YAF2 polyclonal antibody at 1:500 dilutionLane1:Jurkat whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of YAF2 polyclonal antibody at 1:500 dilutionLane1:Jurkat whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)
Related Product Information for anti-YAF2 antibody
Members of the myeloctyomatosis viral oncogene homologue (Myc) family, which include c-Myc, N-Myc and L-Myc, encode transcription factors that play a role in the acceleration of cell cycle progression and cell growth, inhibition of cell differentiation, and initiation of programmed cell death (apoptosis). YY1-associated factor 2, also known as YAF2, is a 180 amino acid protein that binds to c-Myc and inhibits c-Myc-mediated pathways. It also binds to N-Myc, enhancing N-Myc-dependent transcriptional activation. Furthermore, YAF2 interacts with YY1 to antagonize YY1 transactivation of muscle-specific promoters. Localized to the nucleus, YAF2 contains an N-terminal C2-X10-C2 zinc finger and exists as two isoforms.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 20kDa
NCBI Official Full Name
YY1-associated factor 2 isoform 3
UniProt Protein Name
YY1-associated factor 2
Protein Family
UniProt Gene Name
YAF2
UniProt Entry Name
YAF2_HUMAN
Uniprot Description
YAF2: Binds to MYC and inhibits MYC-mediated transactivation. Also binds to MYCN and enhances MYCN-dependent transcriptional activation. Increases calpain 2-mediated proteolysis of YY1 in vitro. Component of the E2F6.com-1 complex, a repressive complex that methylates 'Lys-9' of histone H3, suggesting that it is involved in chromatin-remodeling. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 12q12
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: protein binding; zinc ion binding; transcription coactivator activity; transcription corepressor activity
Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of transcription, DNA-dependent
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Product Notes
The YAF2 yaf2 (Catalog #AAA3006522) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The YAF2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's YAF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the YAF2 yaf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YAF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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