Rabbit PPP1R13B Polyclonal Antibody | anti-PPP1R13B antibody

PPP1R13B Polyclonal Antibody

Cat. Num. AAA3007033

• Gene Names: PPP1R13B; p85; ASPP1; p53BP2-like
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PPP1R13B; Polyclonal Antibody; PPP1R13B Polyclonal Antibody; Apoptosis-stimulating of p53 protein 1; Protein phosphatase 1 regulatory subunit 13B; ASPP1; KIAA0771; anti-PPP1R13B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PPP1R13B polyclonal antibody detects endogenous levels of PPP1R13B protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1090

• Applicable Applications for anti-PPP1R13B antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human PPP1R13B
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PPP1R13B polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysate Lane3:H9C2 whole cell lysate)

Related Product Information for anti-PPP1R13B antibody

ASPP proteins interact with p53 and are responsible for enhancing p53-induced apoptosis but not cell cycle arrest. Inhibition of endogenous ASPP1 (PPP1R13B) function inhibits the apoptotic function of endogenous p53 in response to apoptotic stimuli. ASPP1 amplifies DNA binding and transactivation function of p53 on the promoters of proapoptotic genes in vivo. Expres-sion of ASPP1 is often downregulated in human breast carcinomas expressing wildtype p53, but not in those expressing mutant p53. This research indicates that ASPP1 regulates the tumor suppression function of p53 in vivo. ASPP1 is predominantly a cytoplasmic protein, although some fraction of the polypeptide is nuclear. Defects in PPP1R13B, the gene which encodes ASPP1, may be a cause of breast cancers. The deduced ASPP1 protein contains 1, 090 amino acid residues.

NCBI and Uniprot Product Information

• NCBI GI #: 121114287
• NCBI GeneID: 23368
• NCBI Accession #: NP_056131.2
• NCBI GenBank Nucleotide #: NP_056131.2
• UniProt Accession #: Q96KQ4
• Molecular Weight: ~ 120kDa
• NCBI Official Full Name: apoptosis-stimulating of p53 protein 1
• NCBI Official Synonym Full Names: protein phosphatase 1 regulatory subunit 13B
• NCBI Official Symbol: PPP1R13B
• NCBI Official Synonym Symbols: p85; ASPP1; p53BP2-like
• NCBI Protein Information: apoptosis-stimulating of p53 protein 1
• UniProt Protein Name: Apoptosis-stimulating of p53 protein 1
• Protein Family: Apoptosis-stimulating of p53 protein
• UniProt Gene Name: PPP1R13B
• UniProt Synonym Gene Names: ASPP1; KIAA0771
• UniProt Entry Name: ASPP1_HUMAN

NCBI Description

This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. ASPP proteins are required for the induction of apoptosis by p53-family proteins. They promote DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor. [provided by RefSeq, Jul 2008]