Rabbit anti-Human XRN2 Polyclonal Antibody | anti-XRN2 antibody

XRN2, NT (XRN2, 5'-3' exoribonuclease 2, DHM1-like protein) (Biotin)

Cat. Num. AAA6364129

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: XRN2; Polyclonal Antibody; NT (XRN2; 5'-3' exoribonuclease 2; DHM1-like protein) (Biotin); DHM1-like protein; anti-XRN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-XRN2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: XRN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-76 amino acids from the N-terminal region of human XRN2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(XRN2 Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the XRN2 antibody detected the XRN2 protein (arrow).)

Related Product Information for anti-XRN2 antibody

This gene shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The yeast gene is involved in homologous recombination and RNA metabolism, such as RNA synthesis and RNA trafficking. Complementation studies show that Dhm1 has a similar function in mouse as dhp1. The function of the human gene has not yet been determined. Transcript variants encoding different isoforms have been noted for this gene; however, their full-length nature is not known.

Product Categories/Family for anti-XRN2 antibody

Antibodies; Enzymes

References

Lu, Y., et al. Oncogene 29(7):1041-1049(2010). Yang, X.C., et al. Mol. Cell. Biol. 29(1):31-42(2009). Kaneko, S., et al. Genes Dev. 21(14):1779-1789(2007). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 18860916
• NCBI GeneID: 22803
• NCBI Accession #: NP_036387.2
• NCBI GenBank Nucleotide #: NM_012255.3
• UniProt Accession #: Q9H0D6; Q3L8N4; Q6KGZ9; Q9BQL1; Q9NTW0; Q9NXS6; Q9UL53
• Molecular Weight: 108,582 Da
• NCBI Official Full Name: 5'-3' exoribonuclease 2
• NCBI Official Synonym Full Names: 5'-3' exoribonuclease 2
• NCBI Official Symbol: XRN2
• NCBI Protein Information: 5'-3' exoribonuclease 2; DHP protein; Dhm1-like protein (mouse homolog)
• UniProt Protein Name: 5'-3' exoribonuclease 2
• UniProt Gene Name: XRN2
• UniProt Synonym Gene Names: DHP protein
• UniProt Entry Name: XRN2_HUMAN

NCBI Description

This gene shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The yeast gene is involved in homologous recombination and RNA metabolism, such as RNA synthesis and RNA trafficking. Complementation studies show that Dhm1 has a similar function in mouse as dhp1. The function of the human gene has not yet been determined. Transcript variants encoding different isoforms have been noted for this gene; however, their full-length nature is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

XRN2: Possesses 5'->3' exoribonuclease activity. May promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription. Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.13.-; Ribonuclease; RNA processing; RNA-binding; Cell development/differentiation; Nucleolus

Chromosomal Location of Human Ortholog: 20p11.2-p11.1

Cellular Component: membrane; nucleolus; nucleus

Molecular Function: 5'-3' exonuclease activity; 5'-3' exoribonuclease activity; nuclease activity; zinc ion binding

Biological Process: RNA processing; regulation of transcription, DNA-dependent; RNA metabolic process; RNA catabolic process; spermatogenesis; cell growth; mRNA processing; DNA catabolic process, exonucleolytic; transcription termination