Rabbit anti-Human XRCC5 Polyclonal Antibody | anti-XRCC5 antibody

XRCC5, ID (K439) (XRCC5, G22P2, X-ray repair cross-complementing protein 5, 86kD subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80kD subunit, CTC box-binding factor 85kD subunit, DNA repair protein XRCC5, Ku80

Cat. Num. AAA6364083

• Gene Names: XRCC5; KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: XRCC5; Polyclonal Antibody; ID (K439) (XRCC5; G22P2; X-ray repair cross-complementing protein 5; 86kD subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80kD subunit; CTC box-binding factor 85kD subunit; DNA repair protein XRCC5; Ku80; Ku86; Lupus Ku autoantigen protein p86; Nuclear factor IV; Thyroid-lupus autoantigen; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); anti-XRCC5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human XRCC5.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-XRCC5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 424-450 in the central region of human XRCC5.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in human placenta tissue lysates (35ug/lane) usingMBS6002974. This demonstrates that MBS6002974 detected the XRCC5 protein (arrow).)

Western Blot (WB)

(Western Blot analysis using MBS6002974 at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20ug per lane. Secondary IgG (HRP) Goat Anti-Rabbit at 1:10000 dilution. Predicted band size: 83kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data

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Related Product Information for anti-XRCC5 antibody

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Product Categories/Family for anti-XRCC5 antibody

Antibodies; DNA Replication; Repair

References

1. Gomes, B.C., et al. Oncol. Rep. 24(4):1079-1085(2010). 2. Liu, Y., et al. Carcinogenesis 31(10):1762-1769(2010). 3. Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010). In press:4. Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010). 5. Monsees, G.M., et al. Breast Cancer Res. Treat. (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 10863945
• NCBI GeneID: 7520
• NCBI Accession #: NP_066964
• NCBI GenBank Nucleotide #: NM_021141
• UniProt Accession #: P13010; Q0Z7V0; Q4VBQ5; Q53HH7; Q7M4N0; Q9UCQ0; Q9UCQ1; A8K3X5
• Molecular Weight: 82,705 Da
• NCBI Official Full Name: X-ray repair cross-complementing protein 5
• NCBI Official Synonym Full Names: X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
• NCBI Official Symbol: XRCC5
• NCBI Official Synonym Symbols: KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
• NCBI Protein Information: X-ray repair cross-complementing protein 5; 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku autoantigen,
• UniProt Protein Name: X-ray repair cross-complementing protein 5
• UniProt Gene Name: XRCC5
• UniProt Synonym Gene Names: G22P2; CTC85; CTCBF; TLAA
• UniProt Entry Name: XRCC5_HUMAN

Uniprot Description

Ku80: the 80-kilodalton subunit of the Ku complex, also known as ATP-dependant DNA helicase II. A single stranded DNA-dependent ATP-dependent helicase. It functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70.

Protein type: RNA-binding; DNA-binding; EC 3.6.4.-; Nuclear receptor co-regulator; Helicase; Nucleolus; EC 3.6.1.-

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleoplasm; nuclear telomere cap complex; membrane; nuclear chromosome, telomeric region; nucleolus; plasma membrane; nucleus; cytosol

Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; telomeric DNA binding; damaged DNA binding; double-stranded telomeric DNA binding; 5'-deoxyribose-5-phosphate lyase activity; ATP binding

Biological Process: response to drug; cell proliferation; positive regulation of neurogenesis; transcription, DNA-dependent; viral reproduction; double-strand break repair; positive regulation of interferon type I production; innate immune response; brain development; DNA repair; negative regulation of transcription, DNA-dependent; telomere maintenance; double-strand break repair via nonhomologous end joining; DNA duplex unwinding; DNA recombination