Western Blot (WB)
((0.1ug/ml) staining of Human Thyroid lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat XRCC4-like factor/NHEJ1 Polyclonal Antibody | anti-NHEJ1 antibody
Goat anti-XRCC4-like factor/NHEJ1 Antibody
Immunohistochemistry: In paraffin embedded Human Placenta shows nuclear staining in trophoblasts. Recommended concentration, 2.5-3.8ug/ml.
Western Blot: Approx 38kDa band observed in lysates of Human Skin, Testis and Thyroid gland (calculated size of 33.3kDa according to NP_079058.1). The observed molecular weight corresponds to findings with antibodies from other sources. The 38kDa band was successfully blocked by incubation with the immunizing peptide. Recommended concentration 0.1-0.3ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of Human Thyroid lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Thyroid lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008]
Uniprot Description
NHEJ1: DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary. Interacts with XRCC4 and the XRCC4-LIG4 complex. Binds DNA in a length-dependent manner. Ubiquitously expressed. Belongs to the XLF family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA repair, damage
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: nucleoplasm; nucleus
Molecular Function: DNA binding; protein binding
Biological Process: B cell differentiation; central nervous system development; DNA recombination; double-strand break repair via nonhomologous end joining; positive regulation of ligase activity; response to ionizing radiation; T cell differentiation
Disease: Severe Combined Immunodeficiency With Microcephaly, Growth Retardation, And Sensitivity To Ionizing Radiation
Research Articles on NHEJ1
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Product Notes
The NHEJ1 nhej1 (Catalog #AAA421168) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-XRCC4-like factor/NHEJ1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's XRCC4-like factor/NHEJ1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Placenta shows nuclear staining in trophoblasts. Recommended concentration, 2.5-3.8ug/ml. Western Blot: Approx 38kDa band observed in lysates of Human Skin, Testis and Thyroid gland (calculated size of 33.3kDa according to NP_079058.1). The observed molecular weight corresponds to findings with antibodies from other sources. The 38kDa band was successfully blocked by incubation with the immunizing peptide. Recommended concentration 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the NHEJ1 nhej1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QRPQLSKVKR KKPR. It is sometimes possible for the material contained within the vial of "XRCC4-like factor/NHEJ1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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