Rabbit anti-Human NHEJ1 Polyclonal Antibody | anti-NHEJ1 antibody

NHEJ1, CT (NHEJ1, XLF, Non-homologous end-joining factor 1, Protein cernunnos, XRCC4-like factor) (Biotin)

Cat. Num. AAA6325237

• Gene Names: NHEJ1; XLF
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NHEJ1; Polyclonal Antibody; CT (NHEJ1; XLF; Non-homologous end-joining factor 1; Protein cernunnos; XRCC4-like factor) (Biotin); XRCC4-like factor; anti-NHEJ1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-NHEJ1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NHEJ1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-296 amino acids from the C-terminal region of human NHEJ1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NHEJ1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NHEJ1 antibody detected the NHEJ1 protein (arrow).)

Related Product Information for anti-NHEJ1 antibody

Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.

Product Categories/Family for anti-NHEJ1 antibody

Antibodies; DNA Replication; Repair

References

Malivert, L., et al. J. Biol. Chem. 285(34):26475-26483(2010). Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010). Okada, Y., et al. Hum. Mol. Genet. 19(11):2303-2312(2010). Andres, S.N., et al. Mol. Cell 28(6):1093-1101(2007). Tsai, C.J., et al. Proc. Natl. Acad. Sci. U.S.A. 104(19):7851-7856(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 13376142
• NCBI GeneID: 79840
• NCBI Accession #: NP_079058.1
• NCBI GenBank Nucleotide #: NM_024782.2
• UniProt Accession #: Q9H9Q4; Q4ZFW7; Q6IA64; Q96JS9; B8ZZA4
• Molecular Weight: 35,291 Da
• NCBI Official Full Name: non-homologous end-joining factor 1
• NCBI Official Synonym Full Names: nonhomologous end-joining factor 1
• NCBI Official Symbol: NHEJ1
• NCBI Official Synonym Symbols: XLF
• NCBI Protein Information: non-homologous end-joining factor 1; XRCC4-like factor; protein cernunnos
• UniProt Protein Name: Non-homologous end-joining factor 1
• Protein Family: Non-homologous end-joining factor
• UniProt Gene Name: NHEJ1
• UniProt Synonym Gene Names: XLF
• UniProt Entry Name: NHEJ1_HUMAN

NCBI Description

Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008]

Uniprot Description

NHEJ1: DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary. Interacts with XRCC4 and the XRCC4-LIG4 complex. Binds DNA in a length-dependent manner. Ubiquitously expressed. Belongs to the XLF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding

Biological Process: central nervous system development; B cell differentiation; response to ionizing radiation; positive regulation of ligase activity; double-strand break repair via nonhomologous end joining; DNA recombination; T cell differentiation

Disease: Severe Combined Immunodeficiency With Microcephaly, Growth Retardation, And Sensitivity To Ionizing Radiation