Rabbit anti-Human XRCC4 Polyclonal Antibody | anti-XRCC4 antibody

XRCC4 antibody

Cat. Num. AAA9405136

• Gene Names: XRCC4; SSMED
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: XRCC4; Polyclonal Antibody; XRCC4 antibody; anti-XRCC4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 336

• Applicable Applications for anti-XRCC4 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500-1:3000; Immunohistochemistry: 1:100-1:250; Immunofluorescence: 1:100-1:200
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide contain a sequence corresponding to a region within amino acids 216 and 269 (Q13426) of XRCC4
• Target Name: XRCC4
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample(30 ug whole cell lysate)A: 293TB: A431C: HeLa S310% SDS PAGEPrimary antibody diluted at 1: 1000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded A549 xenograft, using XRCC4 antibody at 1: 100 dilution.)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using XRCC4 antibody at 1: 200 dilution.)

Related Product Information for anti-XRCC4 antibody

The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants. [provided by RefSeq]

Product Categories/Family for anti-XRCC4 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 12408647
• NCBI GeneID: 7518
• NCBI Accession #: NP_071801
• NCBI GenBank Nucleotide #: NM_022406.2
• Molecular Weight: 35,372 Da
• NCBI Official Full Name: DNA repair protein XRCC4 isoform 2
• NCBI Official Synonym Full Names: X-ray repair complementing defective repair in Chinese hamster cells 4
• NCBI Official Symbol: XRCC4
• NCBI Official Synonym Symbols: SSMED
• NCBI Protein Information: DNA repair protein XRCC4
• UniProt Protein Name: DNA repair protein XRCC4
• Protein Family: Xrcc4-like factor
• UniProt Gene Name: XRCC4
• UniProt Entry Name: XRCC4_HUMAN

NCBI Description

The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants. [provided by RefSeq, Sep 2008]

Uniprot Description

XRCC4: Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. Homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex formed by the Ku p70/p86 dimer (XRCC6/XRCC5) and PRKDC. Seems to interact directly with PRKDC but not with the Ku p70/86 dimer. Interacts with XLF/Cernunnos. Interacts with APTX and APLF. Widely expressed. Belongs to the XRCC4 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 5q14.2

Cellular Component: nucleoplasm; DNA ligase IV complex; centrosome; condensed chromosome; DNA-dependent protein kinase complex; nucleus; cytosol; cell junction

Molecular Function: protein C-terminus binding; protein binding; DNA binding; ligase activity

Biological Process: pro-B cell differentiation; positive regulation of neurogenesis; central nervous system development; immunoglobulin V(D)J recombination; viral reproduction; in utero embryonic development; T cell differentiation in the thymus; isotype switching; DNA repair; double-strand break repair via nonhomologous end joining; positive regulation of fibroblast proliferation; double-strand break repair; response to gamma radiation; DNA ligation during DNA repair; negative regulation of neuron apoptosis; positive regulation of ligase activity; response to X-ray

Disease: Short Stature, Microcephaly, And Endocrine Dysfunction