Rabbit anti-Human, mouse XLKD1 Polyclonal Antibody | anti-LYVE1 antibody

XLKD1 Antibody (N-term)

Cat. Num. AAA9202727

• Gene Names: LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: XLKD1; Polyclonal Antibody; XLKD1 Antibody (N-term); Lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE-1; Cell surface retention sequence-binding protein 1; CRSBP-1; Extracellular link domain-containing protein 1; Hyaluronic acid receptor; LYVE1; CRSBP1; HAR; anti-LYVE1 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This XLKD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-77 amino acids from the N-terminal region of human XLKD1.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 46-77
• Sequence Length: 322

• Applicable Applications for anti-LYVE1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(The anti-XLKD1 N-term Pab is used in Western blot to detect XLKD1 in mouse liver tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(XLKD1 Antibody (N-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the XLKD1 antibody detected the XLKD1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of XLKD1 (arrow) using rabbit polyclonal XLKD1 Antibody (C61). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the XLKD1 gene.)

Related Product Information for anti-LYVE1 antibody

One of the key groups of molecules regulating leukocyte and tumour cell migration is the glycosaminoglycan hyaluronan (HA). In inflammation, the exit of leukocytes across vascular endothelium to the underlying tissues involves interactions with cell surface lectin-like receptors on the leukocytes that bind HA on the lumenal surface of the endothelium. During normal tissue homeostasis and after tissue injury, HA is mobilized from these sites through lymphatic vessels to the lymph nodes where it is degraded before entering the circulation for rapid uptake by the liver. Lymphatic vessel endothelial hyaluronan receptor (LYVE)-1 is a major receptor for HA on the lymph vessel wall. LYVE-1 is expressed primarily on lymphatic vessel endothelium and is likely to be involved in regulating the traffic of leucocytes and tumour cells to lymph nodes.

Product Categories/Family for anti-LYVE1 antibody

Cardiovascular

References

Jackson, D.G., Trends Cardiovasc. Med. 13(1):1-7 (2003).
Cursiefen, C., et al., Invest. Ophthalmol. Vis. Sci. 43(7):2127-2135 (2002).
Cunnick, G.H., et al., Biochem. Biophys. Res. Commun. 288(4):1043-1046 (2001).
Mouta Carreira, C., et al., Cancer Res. 61(22):8079-8084 (2001).
Banerji, S., et al., J. Cell Biol. 144(4):789-801 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 40549451
• NCBI GeneID: 10894
• NCBI Accession #: NP_006682.2
• NCBI GenBank Nucleotide #: NM_006691.3
• UniProt Accession #: Q9Y5Y7; Q8TC18; Q9UNF4
• Molecular Weight: 35213
• NCBI Official Full Name: lymphatic vessel endothelial hyaluronic acid receptor 1
• NCBI Official Synonym Full Names: lymphatic vessel endothelial hyaluronan receptor 1
• NCBI Official Symbol: LYVE1
• NCBI Official Synonym Symbols: HAR; XLKD1; LYVE-1; CRSBP-1
• NCBI Protein Information: lymphatic vessel endothelial hyaluronic acid receptor 1
• UniProt Protein Name: Lymphatic vessel endothelial hyaluronic acid receptor 1
• Protein Family: Lymphatic vessel endothelial hyaluronic acid receptor
• UniProt Gene Name: LYVE1
• UniProt Synonym Gene Names: CRSBP1; HAR; XLKD1; LYVE-1; CRSBP-1
• UniProt Entry Name: LYVE1_HUMAN

NCBI Description

This gene encodes a type I integral membrane glycoprotein. The encoded protein acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. [provided by RefSeq, Jul 2008]

Uniprot Description

XLKD1: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: transmembrane receptor activity; hyaluronic acid binding; receptor activity

Biological Process: anatomical structure morphogenesis; glycosaminoglycan metabolic process; transport; cell-matrix adhesion; carbohydrate metabolic process; response to wounding; pathogenesis; cell motility; signal transduction; hyaluronan metabolic process; hyaluronan catabolic process