LYVE-1 recombinant protein

Recombinant human LYVE-1 protein

Cat. Num. AAA201321

• Gene Names: LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: LYVE-1; Recombinant human LYVE-1 protein; Lymphatic vessel endothelial hyaluronan receptor 1; CRSBP-1; HAR; XLKD1; LYVE1; Lymphatic vessel endothelial hyaluronan receptor 1 CRSBP 1; CRSBP1; extracellular link domain containing 1; extracellular link domain-containing 1; hyaluronic acid receptor; LYVE 1; Lymphatic endothelium specific hyaluronan receptor; LYVE-1 recombinant protein

• Host: Baculovirus
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl buffer (pH7.5) containing 10% glycerol
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 25-253aa
• Sequence: LRAEELS IQVSCRIMGI TLVSKKANQQ LNFTEAKEAC RLLGLSLAGK DQVETALKAS FETCSYGWVG DGFVVISRIS PNPKCGKNGV GVLIRKVPVS RQFAAYCYNS SDTWTNSCIP EIITTKDPIF NTQTATQTTE FIVSDSTYSV ASPYSTIPAP TTTPPAPAST SIPRRKKLIC VTEVFMETST MSTETEPFVE NKAAFKNEAA GFGG

• Applicable Applications for LYVE-1 recombinant protein: SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Ala
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for LYVE-1 recombinant protein

LYVE-1 is also known as XLKD1 and may function in lympathic hyaluronan transport and have a role in tumor metastasis. This Protein is a Link domain-containing hyaladherin, a protein capable of binding to hyaluronic acid (HA), homologous to CD44, the main HA receptor. It is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels but is also observed in normal liver blood sinusoids, and embryonic blood vessels. It is selective marker of the lymphatic endothelium, is a surface endocytic receptor for hyaluronan (HA), which is an extracellular glycosaminoglycan involved in cell adhesion and migration. Recombinant human LYVE-1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for LYVE-1 recombinant protein

Cardiovascular; Proteins

References

Chen L. et al. (2005) Invest. Ophthalmol Vis. Sci. 46:4536-4540. Banerji S. et al. (1999) J. Cell Biol. 144:789-801.

NCBI and Uniprot Product Information

• NCBI GI #: 40549451
• NCBI GeneID: 10894
• NCBI Accession #: NP_006682.2
• NCBI GenBank Nucleotide #: NM_006691.3
• UniProt Accession #: Q9Y5Y7; Q8TC18; Q9UNF4
• Molecular Weight: 35,213 Da
• NCBI Official Full Name: lymphatic vessel endothelial hyaluronic acid receptor 1
• NCBI Official Synonym Full Names: lymphatic vessel endothelial hyaluronan receptor 1
• NCBI Official Symbol: LYVE1
• NCBI Official Synonym Symbols: HAR; XLKD1; LYVE-1; CRSBP-1
• NCBI Protein Information: lymphatic vessel endothelial hyaluronic acid receptor 1; cell surface retention sequence binding protein-1; cell surface retention sequence-binding protein 1; extracellular link domain containing 1; extracellular link domain-containing 1; extracellular li
• UniProt Protein Name: Lymphatic vessel endothelial hyaluronic acid receptor 1
• UniProt Gene Name: LYVE1
• UniProt Synonym Gene Names: CRSBP1; HAR; XLKD1; LYVE-1; CRSBP-1
• UniProt Entry Name: LYVE1_HUMAN

NCBI Description

This gene encodes a type I integral membrane glycoprotein. The encoded protein acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. [provided by RefSeq, Jul 2008]

Uniprot Description

XLKD1: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: transmembrane receptor activity; hyaluronic acid binding; receptor activity

Biological Process: anatomical structure morphogenesis; glycosaminoglycan metabolic process; transport; cell-matrix adhesion; carbohydrate metabolic process; response to wounding; pathogenesis; cell motility; signal transduction; hyaluronan metabolic process; hyaluronan catabolic process