Rabbit anti-Human XLalphas Polyclonal Antibody | anti-XLalphas antibody

XLalphas antibody

Cat. Num. AAA9406853

• Gene Names: GNAS; AHO; GSA; GSP; POH; GPSA; NESP; SCG6; SgVI; GNAS1; C20orf45
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: XLalphas; Polyclonal Antibody; XLalphas antibody; anti-XLalphas antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 394

• Applicable Applications for anti-XLalphas antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500-1:3000; Immunohistochemistry: 1:50-1:500
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 164 and 394 of human GNAS
• Target Name: XLalphas
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample (30 ug of whole cell lysate) A: Hep G2 B: Raji 10% SDS PAGE Primary antibody diluted at 1: 1000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Colon ca, using GNAS antibody at 1: 500 dilution.)

Related Product Information for anti-XLalphas antibody

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq]

Product Categories/Family for anti-XLalphas antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 12803767
• NCBI GeneID: 2778
• Molecular Weight: 111,025 Da
• NCBI Official Full Name: GNAS protein
• NCBI Official Synonym Full Names: GNAS complex locus
• NCBI Official Symbol: GNAS
• NCBI Official Synonym Symbols: AHO; GSA; GSP; POH; GPSA; NESP; SCG6; SgVI; GNAS1; C20orf45
• NCBI Protein Information: protein ALEX; protein GNAS; protein SCG6 (secretogranin VI)
• UniProt Protein Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
• UniProt Gene Name: GNAS
• UniProt Synonym Gene Names: GNAS1; GSP
• UniProt Entry Name: GNAS2_HUMAN

NCBI Description

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq, Aug 2012]

Uniprot Description

G-alpha(s): a guanine nucleotide-binding protein of the G12 class of G-alpha proteins. Agonist binding to Gq-coupled receptors may block Akt activation via the release of active G-alpha(q) subunits that inhibit phosphatidylinositol 3-kinase. Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Protein type: G protein, heterotrimeric; G protein; G protein, heterotrimeric alpha G(s); Membrane protein, peripheral; Vesicle; Oncoprotein

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: dendrite; extracellular region; cytosol; ruffle; transport vesicle; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; heterotrimeric G-protein complex; intrinsic to membrane; trans-Golgi network membrane; nucleus

Molecular Function: corticotropin-releasing hormone receptor 1 binding; GTPase activity; GTP binding; metal ion binding; adenylate cyclase activity; insulin-like growth factor receptor binding; ionotropic glutamate receptor binding; protein binding; signal transducer activity; mu-type opioid receptor binding; beta-2 adrenergic receptor binding; G-protein beta/gamma-subunit binding; D1 dopamine receptor binding

Biological Process: developmental growth; positive regulation of osteoclast differentiation; water transport; protein secretion; female pregnancy; G-protein signaling, adenylate cyclase activating pathway; embryonic hindlimb morphogenesis; intracellular transport; positive regulation of cAMP biosynthetic process; tissue homeostasis; DNA methylation; transmembrane transport; dopamine receptor, adenylate cyclase activating pathway; post-embryonic body morphogenesis; endochondral ossification; response to drug; sensory perception of chemical stimulus; negative regulation of multicellular organism growth; multicellular organism growth; sensory perception of smell; adenylate cyclase activation; embryonic cranial skeleton morphogenesis; positive regulation of osteoblast differentiation; cartilage development; energy reserve metabolic process; renal water homeostasis; cAMP biosynthetic process; cognition; blood coagulation; regulation of insulin secretion

Disease: Osseous Heteroplasia, Progressive; Pseudohypoparathyroidism, Type Ic; Pseudopseudohypoparathyroidism; Acth-independent Macronodular Adrenal Hyperplasia; Mccune-albright Syndrome; Pseudohypoparathyroidism, Type Ia; Pseudohypoparathyroidism, Type Ib; Pituitary Adenoma, Growth Hormone-secreting