Rabbit anti-Mouse XIAP Polyclonal Antibody | anti-XIAP antibody

RABBIT ANTI XIAP (C-TERMINAL)

Cat. Num. AAA221825

• Gene Names: XIAP; API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Synonyms: XIAP; Polyclonal Antibody; RABBIT ANTI XIAP (C-TERMINAL); anti-XIAP antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item is specific for human X-chromosome linked inhibitor of apoptosis (XIAP). ; ; XIAP is an endogenous inhibitor of cell death, which functions by suppressing caspases 3, 7, and 9. The protein is also reported to play a a role in the activation of NF kappa B and the regulation of cyclins and cyclin-dependent kinase inhibitors (CDKIs). ; ; Regulators of XIAP activity include XAF-1 and Smac. ; ; This item detects a band of approximately 57KDa in human kidney lysates.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1 mg/ml (varies by lot)
• Sequence Length: 497

• Applicable Applications for anti-XIAP antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Immunohistology - Paraffin: Maximum Dilution: 2ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.; Western Blotting: Minimum Dilution: 0.5ug/ml; Maximum Dilution: 2.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: Synthetic peptide corresponding to a thirteen amino acid sequence with the C-terminal region of human XIAP.; Buffer Solution
• Antiserum Preparation: Antisera to human XIAP were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of human formalin fixed paraffin processed kidney tissue with Rabbit anti Human XIAP at 2ug/ml. (MBS221825))

Testing Data #2

(Western blot analysis of human kidney lysate probed with Rabbit anti Human XIAP (MBS221825) at 0.5(A), 1(B) and 2(C) ug/ml)

Related Product Information for anti-XIAP antibody

MBS221825 is specific for human X-chromosome linked inhibitor of apoptosis (XIAP). XIAP is an endogenous inhibitor of cell death, which functions by suppressing caspases 3, 7, and 9. The protein is also reported to play a a role in the activation of NF kappa B and the regulation of cyclins and cyclin-dependent kinase inhibitors (CDKIs). Regulators of XIAP activity include XAF-1 and Smac. MBS221825 detects a band of approximately 57KDa in human kidney lysates.

NCBI and Uniprot Product Information

• NCBI GI #: 32528299
• NCBI GeneID: 331
• NCBI Accession #: NP_001158.2
• NCBI GenBank Nucleotide #: NM_001167.3
• UniProt Accession #: P98170; Q9NQ14; D3DTF2
• Molecular Weight: 56,685 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase XIAP
• NCBI Official Synonym Full Names: X-linked inhibitor of apoptosis, E3 ubiquitin protein ligase
• NCBI Official Symbol: XIAP
• NCBI Official Synonym Symbols: API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3
• NCBI Protein Information: E3 ubiquitin-protein ligase XIAP; IAP-like protein; X-linked IAP; baculoviral IAP repeat-containing protein 4; inhibitor of apoptosis protein 3
• UniProt Protein Name: E3 ubiquitin-protein ligase XIAP
• Protein Family: XIAP-associated factor
• UniProt Gene Name: XIAP
• UniProt Synonym Gene Names: API3; BIRC4; IAP3; ILP; hILP; IAP-3; hIAP-3; hIAP3; X-linked IAP
• UniProt Entry Name: XIAP_HUMAN

NCBI Description

This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.[provided by RefSeq, Feb 2011]

Uniprot Description

XIAP: an apoptotic suppressor protein. Inhibist apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. Inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. Inhibits cell-death proteases, caspase-3, caspase-7, and perhaps caspase-9.

Protein type: Apoptosis; Ubiquitin conjugating system; Ligase; Ubiquitin ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: nucleoplasm; spindle microtubule; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; zinc ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; ligase activity

Biological Process: Wnt receptor signaling pathway; positive regulation of protein ubiquitination; apoptosis; regulation of BMP signaling pathway; regulation of inflammatory response; protein ubiquitination; negative regulation of caspase activity; response to DNA damage stimulus; copper ion homeostasis; regulation of cell proliferation; negative regulation of apoptosis; regulation of innate immune response

Disease: Lymphoproliferative Syndrome, X-linked, 1; Lymphoproliferative Syndrome, X-linked, 2