Rabbit XEDAR Polyclonal Antibody | anti-XEDAR antibody

RABBIT ANTI HUMAN XEDAR

Cat. Num. AAA221471

• Gene Names: EDA2R; XEDAR; EDAA2R; EDA-A2R; TNFRSF27
• Applications: Western Blot
• Synonyms: XEDAR; Polyclonal Antibody; RABBIT ANTI HUMAN XEDAR; anti-XEDAR antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects human X-linked ectodermal dysplasia receptor (XEDAR), a 33kD type III transmembrane protein of the TNFR (tumor necrosis factor receptor) superfamily. XEDAR is a homologue of the ectodermal dysplasia receptor (EDAR), and binds to an isoform of ectodysplasin termed EDA-A2. Binding of ectodysplasin to XEDAR results in the induction of apoptosis, and is thought to be involved ectodermal organ development. New evidence suggests XEDAR may also be involved in skeletal muscle development. ; Two predominant isoforms of XEDAR exist; XEDAR-s and XEDAR-L, produced by alternative splicing.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 297

• Applicable Applications for anti-XEDAR antibody: Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 33kDa in 293 cell lysates.; Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: Recombinant human XEDAR.; Buffer Solution
• Antiserum Preparation: Antisera to XEDAR were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysates from HEH293 human epithelial kidney cells probed with Rabbit anti Human XEDAR (MBS221471) at 0.5(A), 1(B) and 2(C))

Testing Data #2

(Immunohistochemical staining of human skin with Rabbit anti Human XEDAR (MBS221471))

Related Product Information for anti-XEDAR antibody

MBS221471 detects human X-linked ectodermal dysplasia receptor (XEDAR), a 33kD type III transmembrane protein of the TNFR (tumor necrosis factor receptor) superfamily. XEDAR is a homologue of the ectodermal dysplasia receptor (EDAR), and binds to an isoform of ectodysplasin termed EDA-A2. Binding of ectodysplasin to XEDAR results in the induction of apoptosis, and is thought to be involved ectodermal organ development. New evidence suggests XEDAR may also be involved in skeletal muscle development. Two predominant isoforms of XEDAR exist; XEDAR-s and XEDAR-L, produced by alternative splicing.

NCBI and Uniprot Product Information

• NCBI GI #: 314122209
• NCBI GeneID: 60401
• NCBI Accession #: NP_001186616.1
• NCBI GenBank Nucleotide #: NM_001199687.2
• UniProt Accession #: Q9HAV5; Q5VYX9; Q5VYY0; Q6UWM2; Q8IZA6
• Molecular Weight: 32,959 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 27 isoform 1
• NCBI Official Synonym Full Names: ectodysplasin A2 receptor
• NCBI Official Symbol: EDA2R
• NCBI Official Synonym Symbols: XEDAR; EDAA2R; EDA-A2R; TNFRSF27
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 27; EDA-A2 receptor; X-linked ectodysplasin-A2 receptor; tumor necrosis factor receptor superfamily member XEDAR
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 27
• UniProt Gene Name: EDA2R
• UniProt Synonym Gene Names: TNFRSF27; XEDAR; EDA-A2 receptor
• UniProt Entry Name: TNR27_HUMAN

NCBI Description

EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Mutations in EDA give rise to a clinical syndrome characterized by loss of hair, sweat glands, and teeth. The protein encoded by this gene specifically binds to EDA-A2 isoform. This protein is a type III transmembrane protein of the TNFR (tumor necrosis factor receptor) superfamily, and contains 3 cysteine-rich repeats and a single transmembrane domain but lacks an N-terminal signal peptide. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, May 2011]

Uniprot Description

EDA2R: Receptor for EDA isoform A2, but not for EDA isoform A1. Mediates the activation of the NF-kappa-B and JNK pathways. Activation seems to be mediated by binding to TRAF3 and TRAF6. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq12

Cellular Component: integral to plasma membrane; integral to membrane

Molecular Function: protein binding; tumor necrosis factor receptor activity; receptor activity

Biological Process: epidermis development; tumor necrosis factor-mediated signaling pathway; multicellular organismal development; positive regulation of JNK cascade; activation of NF-kappaB transcription factor