Rabbit anti-Mouse Xaf1 Polyclonal Antibody | anti-Xaf1 antibody

Xaf1, ID (Xaf1, Birc4bp, Xiapaf1, XIAP-associated factor 1, BIRC4-binding protein) (FITC)

Cat. Num. AAA6363888

• Gene Names: Xaf1; Fbox39
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Xaf1; Polyclonal Antibody; ID (Xaf1; Birc4bp; Xiapaf1; XIAP-associated factor 1; BIRC4-binding protein) (FITC); BIRC4-binding protein; anti-Xaf1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-Xaf1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Xaf1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-194 amino acids from the Central region of mouse Xaf1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Xaf1 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Xaf1 antibody detected the Xaf1 protein (arrow).)

Related Product Information for anti-Xaf1 antibody

Xaf1 seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells (By similarity).

Product Categories/Family for anti-Xaf1 antibody

Antibodies; Binding Proteins

References

Bai, Y., et al. J. Biol. Chem. 283(11):6832-6842(2008). Qiao, L., et al. Tumour Biol. 29(2):122-129(2008). Wang, X., et al. Neurobiol. Dis. 16(1):179-189(2004). Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 161333845
• NCBI GeneID: 327959
• NCBI Accession #: NP_001032802.2
• NCBI GenBank Nucleotide #: NM_001037713.4
• UniProt Accession #: Q5NBU8; Q3USK3; Q5NBU6; Q5NBU7; Q5NBU9; B7ZD14
• Molecular Weight: 20,465 Da
• NCBI Official Full Name: XIAP-associated factor 1 isoform 1
• NCBI Official Synonym Full Names: XIAP associated factor 1
• NCBI Official Symbol: Xaf1
• NCBI Official Synonym Symbols: Fbox39
• NCBI Protein Information: XIAP-associated factor 1
• UniProt Protein Name: XIAP-associated factor 1
• Protein Family: XIAP-associated factor
• UniProt Gene Name: Xaf1
• UniProt Synonym Gene Names: Birc4bp; Xiapaf1

Uniprot Description

BIRC4BP: Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti- caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha- induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Tumor suppressor

Chromosomal Location of Human Ortholog: 11|11 B4

Cellular Component: cytoplasm; mitochondrion; nucleus

Molecular Function: metal ion binding

Biological Process: apoptosis; negative regulation of protein complex assembly; response to cytokine