Mouse Glycated Hemoglobin A1c (HbA1c) ELISA Kit | HbA1c elisa kit

Mouse Glycated Hemoglobin A1c (HbA1c) ELISA Kit

• Reactivity: Mouse
• Synonyms: Glycated Hemoglobin A1c (HbA1c); Mouse Glycated Hemoglobin A1c (HbA1c) ELISA Kit; Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbA1c elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of HbA1c. No significant cross-reactivity or interference between HbA1c and analogues was observed.

• Assay Type: Quantitative Competitive
• Samples: Mouse plasma and erythrocyte lysates
• Detection Range: 12.35-1,000ug/mL
• Sensitivity: < 5.25ug/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HbA1c were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HbA1c were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for HbA1c elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to HbA1c has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled HbA1c and unlabeled HbA1c (Standards or samples) with the pre-coated antibody specific to HbA1c. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of HbA1c in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of HbA1c in the sample.

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of HbA1c in mouse plasma and erythrocyte lysates.

References

beta-Adrenergic receptor agonists attenuate pericyte loss in diabetic retinas through Akt activation; FASEB J; 2018 May;32(5):2324-2338.; PMID: 29269397; Jang-Hyuk Yun; Vascular Microenvironment Laboratory, Department of Pharmacology, College of Medicine, Seoul National University, Seoul, South Korea.