Rabbit anti-Human, Mouse WWP2 Polyclonal Antibody | anti-WWP2 antibody

WWP2 Antibody

Cat. Num. AAA9630221

• Gene Names: WWP2; AIP2; WWp2-like
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: WWP2; Polyclonal Antibody; WWP2 Antibody; AIP2; Atrophin 1 interacting protein 2; NEDD4 like E3 ubiquitin protein ligase; WW domain-containing protein 2; Wwp 2; anti-WWP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: WWP2 Antibody detects endogenous levels of WWP2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-WWP2 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human WWP2.
• Conjugation: Unconjugated
• Similarity: The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain. The WW domains mediate interaction with PPxY motif-containing proteins.
• Post Translational Modifications: Autoubiquitinated. Ubiquitinated by the SCF(FBXL15) complex, leading to its degradation by the proteasome.
• Subunit Structure: Interacts with POU5F1, RBP1, EGR2 and SLC11A2 (By similarity). Interacts with SCNN1A, SCNN1B, SCNN1G, WBP1, WBP2 and ATN1. Interacts with ERBB4, NDFIP1 AND NDFIP2. Interacts with ARRDC4. Interacts (via WW domains) with ARRDC1 (via PPxY motifs); ubiquitinates ARRDC1. Interacts (via WW domains) with ARRDC2 and ARRDC3.(Microbial infection) Interacts with adenovirus type 2 PIII.
• Tissue Specificity: Detected in heart, throughout the brain, placenta, lung, liver, muscle, kidney and pancreas. Also detected in spleen and peripheral blood leukocytes.
• Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-WWP2 antibody

Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 32171765
• NCBI GeneID: 11060
• UniProt Accession #: O00308; Q6ZTQ5; Q96CZ2; Q9BWN6; A6NEP1; B2R706; B4DTL5; F5H213; H3BRF3; I3RSG8
• Molecular Weight: Observed MW 105kDa; Calculated MW 35kDa/51kDa/86kDa/98kDa
• NCBI Official Full Name: NEDD4-like E3 ubiquitin-protein ligase WWP2
• NCBI Official Synonym Full Names: WW domain containing E3 ubiquitin protein ligase 2
• NCBI Official Symbol: WWP2
• NCBI Official Synonym Symbols: AIP2; WWp2-like
• NCBI Protein Information: NEDD4-like E3 ubiquitin-protein ligase WWP2; atrophin-1 interacting protein 2
• UniProt Protein Name: NEDD4-like E3 ubiquitin-protein ligase WWP2
• Protein Family: NEDD4-like E3 ubiquitin-protein ligase
• UniProt Gene Name: WWP2
• UniProt Synonym Gene Names: AIP2
• UniProt Entry Name: WWP2_HUMAN

NCBI Description

This gene encodes a member of the Nedd4 family of E3 ligases, which play an important role in protein ubiquitination. The encoded protein contains four WW domains and may play a role in multiple processes including chondrogenesis and the regulation of oncogenic signaling pathways via interactions with Smad proteins and the tumor suppressor PTEN. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 10. [provided by RefSeq, Jul 2012]

Uniprot Description

WWP2: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation- induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.

Protein type: Ubiquitin conjugating system; EC 6.3.2.-; Ligase; EC 6.3.2.19; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: membrane; cytoplasm; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; ubiquitin-protein ligase activity; transcription factor binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; entry of virus into host cell; regulation of membrane potential; protein autoubiquitination; negative regulation of transcription factor activity; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; protein modification process; negative regulation of protein transport; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transporter activity