POLR1C recombinant protein

POLR1C, 1-349aa, Human, His tag, E Coli

Cat. Num. AAA205665

• Gene Names: POLR1C; AC40; RPA5; TCS3; HLD11; RPA39; RPA40; RPAC1; RPC40
• Applications: SDS-Page
• Purity: > 80% by SDS-PAGE
• Synonyms: POLR1C; 1-349aa; Human; His tag; E Coli; DNA-directed RNA polymerases I and III subunit RPAC1; RP3-337H4.4; RPA39; RPA40; RPA5; RPAC1; TCS3; POLR1C recombinant protein

• Host: E Coli
• Purity/Purification: > 80% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAASQAV EEMRSRVVLG EFGVRNVHTT DFPGNYSGYD DAWDQDRFEK NFRVDVVHMD ENSLEFDMVG IDAAIANAFR RILLAEVPTM AVEKVLVYNN TSIVQDEILA HRLGLIPIHA DPRLFEYRNQ GDEEGTEIDT LQFRLQVRCT RNPHAAKDSS DPNELYVNHK VYTRHMTWIP LGNQADLFPE GTIRPVHDDI LIAQLRPGQE IDLLMHCVKG IGKDHAKFSP VATASYRLLP DITLLEPVEG EAAEELSRCF SPGVIEVQEV QGKKVARVAN PRLDTFSREI FRNEKLKKVV RLARVRDHYI FSVESTGVLP PDVLVSEAIK VLMGKCRRFL DELDAVQMD
• Sequence Length: 346

• Applicable Applications for POLR1C recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for POLR1C recombinant protein

POLR1C is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Defects in this gene have been associated with Treacher Collins syndrome (TCS). Recombinant human POLR1C protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for POLR1C recombinant protein

Epigenomics (Transcription & Translation)

References

Hu P., et al (2002). Mol. Cell. Biol. 22:8044-8055; Dauwerse J.G., et al (2011). Nat. Genet. 43:20-22;

NCBI and Uniprot Product Information

• NCBI GI #: 42560246
• NCBI GeneID: 9533
• NCBI Accession #: NP_976035
• NCBI GenBank Nucleotide #: NM_203290.3
• UniProt Accession #: O15160; O75395; Q5JTE3
• Molecular Weight: 41.6 kDa (369aa) confirmed by MALDI-TOF
• NCBI Official Full Name: DNA-directed RNA polymerases I and III subunit RPAC1 isoform 1
• NCBI Official Synonym Full Names: RNA polymerase I subunit C
• NCBI Official Symbol: POLR1C
• NCBI Official Synonym Symbols: AC40; RPA5; TCS3; HLD11; RPA39; RPA40; RPAC1; RPC40
• NCBI Protein Information: DNA-directed RNA polymerases I and III subunit RPAC1
• UniProt Protein Name: DNA-directed RNA polymerases I and III subunit RPAC1
• Protein Family: DNA-directed RNA polymerases
• UniProt Gene Name: POLR1C
• UniProt Synonym Gene Names: POLR1E; DNA-directed RNA polymerase I subunit C; RNA polymerases I and III subunit AC1; RPA40

NCBI Description

The protein encoded by this gene is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Mutations in this gene have been associated with Treacher Collins syndrome (TCS) and hypomyelinating leukodystrophy 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft ().