Rabbit anti-Human WRNIP1 Polyclonal Antibody | anti-WRNIP1 antibody

WRNIP1, NT (WRNIP1, WHIP, ATPase WRNIP1, Werner helicase-interacting protein 1) (APC)

Cat. Num. AAA6363776

• Gene Names: WRNIP1; WHIP; bA420G6.2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: WRNIP1; Polyclonal Antibody; NT (WRNIP1; WHIP; ATPase WRNIP1; Werner helicase-interacting protein 1) (APC); Werner helicase-interacting protein 1; anti-WRNIP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-WRNIP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: WRNIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human WRNIP1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(WRNIP1 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the WRNIP1 antibody detected the WRNIP1 protein (arrow).)

Related Product Information for anti-WRNIP1 antibody

Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging. The protein encoded by this gene interacts with the N-terminal portion of Werner protein containing the exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene may influence the aging process. Two transcript variants encoding different isoforms have been isolated for this gene.

Product Categories/Family for anti-WRNIP1 antibody

Antibodies; DNA Replication; Repair

References

Kaur, S., et al. Cell Cycle 9(15):3106-3111(2010). Yoshimura, A., et al. Genes Genet. Syst. 84(2):171-178(2009). Crosetto, N., et al. J. Biol. Chem. 283(50):35173-35185(2008). Mano, Y., et al. Cancer Sci. 98(12):1902-1913(2007). Bish, R.A., et al. J. Biol. Chem. 282(32):23184-23193(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 18426902
• NCBI GeneID: 56897
• NCBI Accession #: NP_064520.2
• NCBI GenBank Nucleotide #: NM_020135.2
• UniProt Accession #: Q96S55; Q53EP6; Q59ET8; Q5W0E2; Q5W0E4; Q8WV26; Q9H681; Q9NRJ6; B2RDB0
• Molecular Weight: 31,339 Da
• NCBI Official Full Name: ATPase WRNIP1 isoform 1
• NCBI Official Synonym Full Names: Werner helicase interacting protein 1
• NCBI Official Symbol: WRNIP1
• NCBI Official Synonym Symbols: WHIP; bA420G6.2
• NCBI Protein Information: ATPase WRNIP1; putative helicase RUVBL; Werner helicase-interacting protein 1
• UniProt Protein Name: ATPase WRNIP1
• Protein Family: ATPase
• UniProt Gene Name: WRNIP1
• UniProt Entry Name: WRIP1_HUMAN

NCBI Description

Werner's syndrome is a rare autosomal recessive disorder characterized by accelerated aging that is caused by defects in the Werner syndrome ATP-dependent helicase gene (WRN). The protein encoded by this gene interacts with the exonuclease-containing N-terminal portion of the Werner protein. This protein has a ubiquitin-binding zinc-finger domain in the N-terminus, an ATPase domain, and two leucine zipper motifs in the C-terminus. It has sequence similarity to replication factor C family proteins and is conserved from E. coli to human. This protein likely accumulates at sites of DNA damage by interacting with polyubiquinated proteins and also binds to DNA polymerase delta and increases the initiation frequency of DNA polymerase delta-mediated DNA synthesis. This protein also interacts with nucleoporins at nuclear pore complexes. Two transcript variants encoding different isoforms have been isolated for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

WRNIP1: a modulator for initiation or restart of DNA polymerase delta-mediated DNA synthesis. Its ATPase activity may sense DNA ends and regulate the extent of polymerase stimulation.

Protein type: Helicase; EC 3.6.1.3

Chromosomal Location of Human Ortholog: 6p25.2

Cellular Component: membrane; perinuclear region of cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding; metal ion binding; ATPase activity; ATP binding

Biological Process: regulation of DNA replication initiation; DNA synthesis during DNA repair; DNA replication