Western Blot (WB)
(Western Blot analysis of West Nile Virus glycoprotein E in (A) untransfected and (B) transfected cells using MBS628104 at 1ug/ml.)
Rabbit West Nile Virus, Envelope Polyclonal Antibody
West Nile Virus, Envelope (West Nile Virus E Protein, WNV Envelope Protein, Envelope Protein, Genome Polyprotein, Major Envelope Protein E, WNVgp1)
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
West Nile Virus; Envelope; Polyclonal Antibody; Envelope (West Nile Virus E Protein; WNV Envelope Protein; Envelope Protein; Genome Polyprotein; Major Envelope Protein E; WNVgp1); Anti -West Nile Virus; anti-West Nile Virus; Envelope antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes West Nile Virus envelope glycoprotein E. Crossreacts with many species.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in 0.05% sodium azide.
Applicable Applications for anti-West Nile Virus, Envelope antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1-3ug/ml
Immunohistochemistry (Frozen): 1ug/ml
Immunohistochemistry (Paraffin-embedded): 1ug/ml Protease 3min RT is recommended
Dilution: Western Blot: 1-3ug/ml
Immunohistochemistry (Frozen): 1ug/ml
Immunohistochemistry (Paraffin-embedded): 1ug/ml Protease 3min RT is recommended
Immunogen
Synthetic peptide corresponding to 73-86, CPTMGEAHNEKRAD of West Nile virus envelope glycoprotein (NC_001563).
Positive Control
Transfected cells and WNV-Envelope protein in bird liver.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of West Nile Virus glycoprotein E in (A) untransfected and (B) transfected cells using MBS628104 at 1ug/ml.)
Western Blot (WB)
(Western Blot analysis of West Nile Virus glycoprotein E in (A) untransfected and (B) transfected cells using MBS628104 at 1ug/ml.)
Immunohistochemistry (IHC)
(IHC staining of WNV-Envelope protein in bird liver using MBS628104 at 1ug/ml (incubation time: 30 mins.). Ventana Protease 2 was used for antigen retrieval; incubation with the enzyme was done for three mins. at room temperature with a parafilm coverslip (using an autostainer which keeps residual fluid on the slides during every step, so the final concentration is approximately 2.5 times more dilute). Biotinylated secondary antibody, Streptavidin-AKP and Fast Red chromagen were used for visualization. Image and protocol courtesy of Angela Ellis, University of Georgia College of Veterinary Medicine.)
Immunohistochemistry (IHC)
(IHC staining of WNV-Envelope protein in bird liver using MBS628104 at 1ug/ml (incubation time: 30 mins.). Ventana Protease 2 was used for antigen retrieval; incubation with the enzyme was done for three mins. at room temperature with a parafilm coverslip (using an autostainer which keeps residual fluid on the slides during every step, so the final concentration is approximately 2.5 times more dilute). Biotinylated secondary antibody, Streptavidin-AKP and Fast Red chromagen were used for visualization. Image and protocol courtesy of Angela Ellis, University of Georgia College of Veterinary Medicine.)
Related Product Information for anti-West Nile Virus, Envelope antibody
West Nile Virus (WNV) is a member of the Flaviviridae, a plus-stranded virus family that includes St. Louis encephalitis virus, yellow fever virus, and Dengue virus. WNV was initially isolated in 1937 in the West Nile region of Uganda and has become prevalent in Africa, Asia, and Europe. It has rapidly spread across the United States with cases being observed in every continental state (1). Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope embedded with envelope and matrix proteins (1). While the viral core protein is thought to contribute to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway (2), the highly glycosylated envelope protein plays a major role for WNV entry into target cells as this entry can be inhibited by using a recombinant domain III from the envelope glycoprotein (3). The WNV receptor has recently been identified as alpha v beta 3 integrin (4).
Product Categories/Family for anti-West Nile Virus, Envelope antibody
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Product Notes
The West Nile Virus, Envelope (Catalog #AAA628104) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's West Nile Virus, Envelope can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 1-3ug/ml Immunohistochemistry (Frozen): 1ug/ml Immunohistochemistry (Paraffin-embedded): 1ug/ml Protease 3min RT is recommended. Researchers should empirically determine the suitability of the West Nile Virus, Envelope for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "West Nile Virus, Envelope, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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