Rabbit WNT4 Polyclonal Antibody | anti-WNT4 antibody

Anti-WNT4 Antibody (C-Terminus) IHC-plus

Cat. Num. AAA249702

• Gene Names: WNT4; WNT-4; SERKAL
• Reactivity: Reactivity: Human (tested or 100% immunogen sequence identity); Predicted: Mouse, Rat (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot
• Purity: Ammonium sulfate precipitation
• Synonyms: WNT4; Polyclonal Antibody; Anti-WNT4 Antibody (C-Terminus) IHC-plus; WNT4 Antibody; WNT-4 Antibody; Protein Wnt-4 Antibody; SERKAL Antibody; anti-WNT4 antibody

• Host: Rabbit
• Reactivity: Reactivity: Human (tested or 100% immunogen sequence identity); Predicted: Mouse, Rat (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: This WNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-269 amino acids from the C-terminal region of human WNT4
• Purity/Purification: Ammonium sulfate precipitation
• Form/Format: PBS, 0.09% sodium azide
• Sequence Positions: 242-269

• Applicable Applications for anti-WNT4 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P (1:100); WB (1:1000); Optimal dilution to be determined by the researcher.
• Immunogen Species: WNT4 antibody was raised against human
• Family/Subfamily: WNT/not assigned-WNT
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months.; For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-WNT4 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Western Blot (WB)

(Western blot of WNT4 antibody in 293 cell line lysates (35 ug/lane). WNT4 (arrow) was detected using the purified antibody.)

Related Product Information for anti-WNT4 antibody

WNT4 Antibody, WNT-4 Antibody, Protein Wnt-4 Antibody, SERKAL Antibody Description: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat.

NCBI and Uniprot Product Information

• NCBI GI #: 17402922
• NCBI GeneID: 54361
• NCBI Accession #: NP_110388.2
• NCBI GenBank Nucleotide #: NM_030761.4
• UniProt Accession #: P56705; Q5TZQ0; Q96T81; Q9BXF5; Q9H1J8; Q9UJM2; B4DJF9
• NCBI Official Full Name: protein Wnt-4
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family member 4
• NCBI Official Symbol: WNT4
• NCBI Official Synonym Symbols: WNT-4; SERKAL
• NCBI Protein Information: protein Wnt-4
• UniProt Protein Name: Protein Wnt-4
• UniProt Gene Name: WNT4
• UniProt Entry Name: WNT4_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT4: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Overexpression may be associated with abnormal proliferation in human breast tissue. Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome); also called Mayer- Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls. Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL); also known as SERKAL syndrome. Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL). Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.23-p35.1

Cellular Component: cell surface; cytoplasm; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane; proteinaceous extracellular matrix

Molecular Function: frizzled binding; receptor agonist activity; transcription corepressor activity

Biological Process: adrenal gland development; androgen biosynthetic process; cell fate commitment; cellular response to starvation; embryonic epithelial tube formation; epithelial to mesenchymal transition; female gonad development; female sex determination; immature T cell proliferation in the thymus; kidney development; liver development; male gonad development; negative regulation of cell differentiation; negative regulation of cell migration; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of transcription, DNA-dependent; neuron differentiation; oocyte development; positive regulation of aldosterone biosynthetic process; positive regulation of bone mineralization; positive regulation of collagen biosynthetic process; positive regulation of focal adhesion formation; positive regulation of GTPase activity; positive regulation of meiosis; positive regulation of osteoblast differentiation; positive regulation of stress fiber formation; positive regulation of transcription, DNA-dependent; regulation of cell-cell adhesion; smooth muscle cell differentiation; somatotropin secreting cell differentiation; thyroid stimulating hormone secreting cell differentiation; ureteric bud branching; Wnt receptor signaling pathway through beta-catenin

Disease: 46,xx Sex Reversal With Dysgenesis Of Kidneys, Adrenals, And Lungs; Mullerian Aplasia And Hyperandrogenism