Western Blot (WB)
(Figure 1. Western blot analysis of Wnt2b using anti-Wnt2b antibody (MBS1753675).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: human Jurkat whole cell lysatesLane 4: human A549 whole cell lysatesLane 5: human Raji whole cell lysatesLane 6: human SW620 whole cell lysatesLane 7: human SKOV3 whole cell lysatesLane 8: rat PC-12 whole cell lysatesLane 9: mouse Neuro-2a whole cell lysatesLane 10: mouse ANA-1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Wnt2b antigen affinity purified polyclonal antibody (Catalog # MBS1753675) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Wnt2b at approximately 60KD. The expected band size for Wnt2b is at 60KD. )
Rabbit Wnt2b Polyclonal Antibody | anti-WNT2B antibody
Anti-Wnt2b Antibody
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of Wnt2b using anti-Wnt2b antibody (MBS1753675).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: human Jurkat whole cell lysatesLane 4: human A549 whole cell lysatesLane 5: human Raji whole cell lysatesLane 6: human SW620 whole cell lysatesLane 7: human SKOV3 whole cell lysatesLane 8: rat PC-12 whole cell lysatesLane 9: mouse Neuro-2a whole cell lysatesLane 10: mouse ANA-1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Wnt2b antigen affinity purified polyclonal antibody (Catalog # MBS1753675) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Wnt2b at approximately 60KD. The expected band size for Wnt2b is at 60KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of Wnt2b using anti-Wnt2b antibody (MBS1753675).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: human Jurkat whole cell lysatesLane 4: human A549 whole cell lysatesLane 5: human Raji whole cell lysatesLane 6: human SW620 whole cell lysatesLane 7: human SKOV3 whole cell lysatesLane 8: rat PC-12 whole cell lysatesLane 9: mouse Neuro-2a whole cell lysatesLane 10: mouse ANA-1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Wnt2b antigen affinity purified polyclonal antibody (Catalog # MBS1753675) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Wnt2b at approximately 60KD. The expected band size for Wnt2b is at 60KD. )
2. Ober, E. A., Verkade, H., Field, H. A., Stainier, D. Y. R. Mesodermal Wnt2b signalling positively regulates liver specification. Nature 442: 688-691, 2006.
3. Wolda, S. L., Moon, R. T. Cloning and developmental expression in Xenopus laevis of seven additional members of the Wnt family. Oncogene 7: 1941-1947, 1992.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as carcinogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Uniprot Description
WNT2B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 1p13
Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region
Molecular Function: frizzled binding
Biological Process: neuron differentiation; lens development in camera-type eye; Wnt receptor signaling pathway; cell fate commitment; male gonad development; chondrocyte differentiation; Wnt receptor signaling pathway through beta-catenin; forebrain regionalization; cellular response to starvation
Research Articles on WNT2B
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Product Notes
The WNT2B wnt2b (Catalog #AAA1753675) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Wnt2b Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Wnt2b can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human, Mouse, Rat| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the WNT2B wnt2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wnt2b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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