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Western Blot (WB) (Western blot (WB) analysis of WNT16 polyclonal antibody at 1:500 dilutionLaneA: SP2/0 whole cell lysateLaneB: The Spleen tissue lysate of MouseLaneC: The Spleen tissue lysate of RatLaneD: HEK293T whole cell lysateLaneE: Panc1 whole cell lysate)

Rabbit WNT16 Polyclonal Antibody | anti-WNT16 antibody

WNT16 polyclonal antibody

Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Synonyms
WNT16; Polyclonal Antibody; WNT16 polyclonal antibody; Protein Wnt-16; anti-WNT16 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
WNT16 polyclonal antibody detects endogenous levels of WNT16 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-WNT16 antibody
Western Blot (WB)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:200
Immunogen
Synthetic peptide, corresponding to Human WNT16
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of WNT16 polyclonal antibody at 1:500 dilutionLaneA: SP2/0 whole cell lysateLaneB: The Spleen tissue lysate of MouseLaneC: The Spleen tissue lysate of RatLaneD: HEK293T whole cell lysateLaneE: Panc1 whole cell lysate)

Western Blot (WB) (Western blot (WB) analysis of WNT16 polyclonal antibody at 1:500 dilutionLaneA: SP2/0 whole cell lysateLaneB: The Spleen tissue lysate of MouseLaneC: The Spleen tissue lysate of RatLaneD: HEK293T whole cell lysateLaneE: Panc1 whole cell lysate)
Related Product Information for anti-WNT16 antibody
The Wnt genes encode a family of secreted extracellular signaling glycoproteins, which function in a variety of important developmental processes such as regulation of cell growth and differentiation. Wnt proteins also play roles in carcinogenesis. Wnt-14, rather than Wnt-15, is preferentially expressed in various types of human cancer. Wnt-15 is expressed in fetal and adult kidney and is most homologous to Wnt-14. Wnt-16, another member in the Wnt family, has two mRNA isoforms, Wnt-16a and Wnt-16b. These isoforms differ in the composition of their 5'UTR and first exon, which results in differential expression. Wnt-16a is expressed only in pancreas, whereas Wnt-16b is highly expressed in adult kidney, placenta, brain, heart and spleen, but not in bone marrow. However, Wnt-16 transcripts are present in bone marrow and cell lines derived from pre-B acute lymphoblastoid leukemia patients carrying the E2A-Pbx1 hybrid gene. Thus, Wnt-16 is a downstream target of E2APbx1, and the Wnt-16-mediated autocrine growth mechanism may contribute to the development of t(1;19) pre-B acute lymphoblastoid leukemias.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,447 Da
NCBI Official Full Name
protein Wnt-16 isoform 2
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 16
NCBI Official Symbol
WNT16
NCBI Protein Information
protein Wnt-16; wingless-type MMTV integration site family member 16b
UniProt Protein Name
Protein Wnt-16
Protein Family
UniProt Gene Name
WNT16
UniProt Entry Name
WNT16_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It contains two transcript variants diverging at the 5' termini. These two variants are proposed to be the products of separate promoters and not to be splice variants from a single promoter. They are differentially expressed in normal tissues, one of which (variant 2) is expressed at significant levels only in the pancreas, whereas another one (variant 1) is expressed more ubiquitously with highest levels in adult kidney, placenta, brain, heart, and spleen. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT16: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: extracellular space; proteinaceous extracellular matrix; cytoplasm; extracellular region

Molecular Function: frizzled binding

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; keratinocyte differentiation; neuron differentiation; Wnt receptor signaling pathway; cell fate commitment; positive regulation of JNK cascade; keratinocyte proliferation; bone remodeling

Research Articles on WNT16

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Product Notes

The WNT16 wnt16 (Catalog #AAA3014953) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WNT16 polyclonal antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's WNT16 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500~1:1000 IHC: 1:50~1:200. Researchers should empirically determine the suitability of the WNT16 wnt16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WNT16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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