Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (WNT16 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the WNT16 antibody detected the WNT16 protein (arrow).)

Rabbit anti-Human WNT16 Polyclonal Antibody | anti-WNT16 antibody

WNT16, CT (WNT16, Protein Wnt-16) (AP)

Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
WNT16; Polyclonal Antibody; CT (WNT16; Protein Wnt-16) (AP); Protein Wnt-16; anti-WNT16 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-WNT16 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
WNT16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-265 amino acids from the C-terminal region of human WNT16.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(WNT16 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the WNT16 antibody detected the WNT16 protein (arrow).)

Western Blot (WB) (WNT16 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the WNT16 antibody detected the WNT16 protein (arrow).)

Immunohistochemistry (IHC)

(WNT16 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WNT16 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (WNT16 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WNT16 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-WNT16 antibody
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It contains two transcript variants diverging at the 5' termini. These two variants are proposed to be the products of separate promoters and not to be splice variants from a single promoter. They are differentially expressed in normal tissues, one of which (variant 2) is expressed at significant levels only in the pancreas, whereas another one (variant 1) is expressed more ubiquitously with highest levels in adult kidney, placenta, brain, heart, and spleen.
Product Categories/Family for anti-WNT16 antibody
References
Binet, R., et al. Cancer Res. 69(24):9183-9191(2009). Memarian, A., et al. Leuk. Lymphoma 50(12):2061-2070(2009). Nygren, M.K., et al. Exp. Hematol. 37(2):225-233(2009). Teh, M.T., et al. J. Cell. Sci. 120 (PT 2), 330-339 (2007): Casagrande, G., et al. Haematologica 91(6):765-771(2006).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein Wnt-16 isoform 1
NCBI Official Synonym Full Names
Wnt family member 16
NCBI Official Symbol
WNT16
NCBI Protein Information
protein Wnt-16
UniProt Protein Name
Protein Wnt-16
Protein Family
UniProt Gene Name
WNT16
UniProt Entry Name
WNT16_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It contains two transcript variants diverging at the 5' termini. These two variants are proposed to be the products of separate promoters and not to be splice variants from a single promoter. They are differentially expressed in normal tissues, one of which (variant 2) is expressed at significant levels only in the pancreas, whereas another one (variant 1) is expressed more ubiquitously with highest levels in adult kidney, placenta, brain, heart, and spleen. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT16: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: extracellular space; proteinaceous extracellular matrix; cytoplasm; extracellular region

Molecular Function: frizzled binding

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; keratinocyte differentiation; neuron differentiation; Wnt receptor signaling pathway; cell fate commitment; positive regulation of JNK cascade; keratinocyte proliferation; bone remodeling

Research Articles on WNT16

Similar Products

Product Notes

The WNT16 wnt16 (Catalog #AAA6363687) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WNT16, CT (WNT16, Protein Wnt-16) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WNT16 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the WNT16 wnt16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WNT16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.