Rabbit anti-Human WNT-1 Polyclonal Antibody | anti-WNT-1 antibody

WNT-1 (WNT-1 Proto-Oncogene Protein, INT1, WNT1) (MaxLight 550)

Cat. Num. AAA6444508

• Gene Names: WNT1; INT1; OI15; BMND16
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: WNT-1; Polyclonal Antibody; WNT-1 (WNT-1 Proto-Oncogene Protein; INT1; WNT1) (MaxLight 550); anti-WNT-1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Wnt-1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight550.

• Applicable Applications for anti-WNT-1 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: IHC-P: paraffin, requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; FLISA: 0.5-2.0ug/ml; WB: 0.1-0.2ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to 343aa from human Wnt-1.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: May be stored at -20 degree C. Stable for 12 months after receipt at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human WNT-1 following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Product Categories/Family for anti-WNT-1 antibody

Antibodies; Wnt Proteins

References

1. Brown, A.M.C. et al. (1987) Identification of protein products encoded by the proto-oncogene int-1. Mol. Cell. Biol. 7: 3971-3977. 2. Maguschak, K.A. et al. (2011) Wnt Signaling in Amygdala-Dependent Learning and Memory J. Neuroscience, 31: 13057-67.

NCBI and Uniprot Product Information

• NCBI GI #: 4885655
• NCBI GeneID: 7471
• NCBI Accession #: NP_005421.1
• NCBI GenBank Nucleotide #: NM_005430.3
• UniProt Accession #: P04628; Q5U0N2
• Molecular Weight: 40,982 Da
• NCBI Official Full Name: proto-oncogene Wnt-1
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 1
• NCBI Official Symbol: WNT1
• NCBI Official Synonym Symbols: INT1; OI15; BMND16
• NCBI Protein Information: proto-oncogene Wnt-1; proto-oncogene Int-1 homolog; wingless-type MMTV integration site family, member 1 (oncogene INT1)
• UniProt Protein Name: Proto-oncogene Wnt-1
• Protein Family: Protein
• UniProt Gene Name: WNT1
• UniProt Synonym Gene Names: INT1
• UniProt Entry Name: WNT1_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT1: Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; Oncoprotein; Cell development/differentiation

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; Golgi lumen; endoplasmic reticulum lumen; cytoplasm; extracellular region; plasma membrane

Molecular Function: protein domain specific binding; frizzled binding; cytokine activity; receptor agonist activity

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; ubiquitin-dependent SMAD protein catabolic process; diencephalon development; negative regulation of BMP signaling pathway; neuron differentiation; cerebellum formation; positive regulation of fibroblast proliferation; cell-cell signaling; midbrain development; positive regulation of cell proliferation; midbrain-hindbrain boundary maturation during brain development; response to wounding; positive regulation of Notch signaling pathway; myoblast fusion; neuron fate determination; inner ear morphogenesis; organ regeneration; cell fate commitment; Wnt receptor signaling pathway; embryonic axis specification; DNA damage response, signal transduction; negative regulation of fat cell differentiation; central nervous system morphogenesis; ureteric bud branching; forebrain anterior/posterior pattern formation; spinal cord association neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell-cell adhesion; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; Spemann organizer formation

Disease: Bone Mineral Density Quantitative Trait Locus 16; Osteogenesis Imperfecta, Type Xv