Western Blot (WB)
(Gel: 6%SDS-PAGE Lysate: 40 μ g Lane 1-2: MCF7 cells, 293T cells, Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 minutes)
Rabbit anti-Human WNK3 Polyclonal Antibody | anti-WNK3 antibody
WNK3 Antibody
Immunohistochemistry: 1:30-1:150
Western Blot (WB)
(Gel: 6%SDS-PAGE Lysate: 40 μ g Lane 1-2: MCF7 cells, 293T cells, Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 minutes)
Western Blot (WB)
(Gel: 6%SDS-PAGE Lysate: 40 μ g Lane 1-2: MCF7 cells, 293T cells, Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 minutes)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/20.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/20.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using at dilution 1/20.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using at dilution 1/20.)
NCBI and Uniprot Product Information
Uniprot Description
WNK3: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Phosphorylates WNK4. Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.11.1; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Wnk family
Chromosomal Location of Human Ortholog: Xp11.22
Cellular Component: adherens junction; cytoplasm; cytosol; tight junction
Molecular Function: ATP binding; chloride channel inhibitor activity; protein binding; protein kinase activity; protein serine/threonine kinase activity
Biological Process: negative regulation of apoptosis; positive regulation of calcium ion transport; positive regulation of ion transmembrane transporter activity; protein amino acid autophosphorylation; protein amino acid phosphorylation
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Product Notes
The WNK3 wnk3 (Catalog #AAA9415175) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WNK3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WNK3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:200-1:1000 Immunohistochemistry: 1:30-1:150. Researchers should empirically determine the suitability of the WNK3 wnk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WNK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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