Rabbit WNK2 Polyclonal Antibody | anti-WNK2 antibody

WNK2 Polyclonal Antibody

Cat. Num. AAA3007306

• Gene Names: WNK2; PRKWNK2; NY-CO-43; SDCCAG43; P/OKcl.13
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: WNK2; Polyclonal Antibody; WNK2 Polyclonal Antibody; Serine/threonine-protein kinase WNK2 (EC: 2.7.11.1); Antigen NY-CO-43; Protein kinase lysine-deficient 2; Protein kinase with no lysine 2; Serologically defined colon cancer antigen 43; KIAA1760; PRKWNK2; SDCCAG43; anti-WNK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: WNK2 polyclonal antibody detects endogenous levels of WNK2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 2297

• Applicable Applications for anti-WNK2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human WNK2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of WNK2 polyclonal antibody at 1:500Line1:Hela whole cell lysateLine2:H9C2 whole cell lysateLine3:Raw264. 7 whole cell lysate)

Related Product Information for anti-WNK2 antibody

The protein kinase superfamily contains over a thousand proteins in 57 subfamilies that all share a catalytic core of 250-300 amino acids organized in two domains. WNK, for "with no lysine (K)", kinases are serine-threonine protein kinases that contain a cysteine residue in place of a lysine residue in a family of proteins that traditionally contain a lysine following a short string of hydrophobic residues. WNK kinases contain a lysine upstream of the traditional position, within a glycine string. This lysine functions as an anchor and orients ATP through interactions with the å and ? phosphoryl groups. The catalytic domains of WNK2, WNK3 and WNK4 are 95% homologous to WNK1. The human WNK1 gene encodes a 2, 382 amino acid protein that is primarily expressed in heart, kidney, muscle and distal nephron. The human WNK3 gene encodes a protein that is primarily expressed in brain; the human WNK4 gene encodes a 1, 243 amino acid protein that is expressed in kidney. Aberrant function of WNK kinases and their associated signaling pathways are implicated in hypertension, increased renal salt reabsorption and impaired K+ and H+ excretion.

NCBI and Uniprot Product Information

• NCBI GI #: 41688799
• NCBI GeneID: 65268
• UniProt Accession #: Q9Y3S1
• Molecular Weight: ~ 234kDa
• NCBI Official Full Name: Serine/threonine-protein kinase WNK2
• NCBI Official Synonym Full Names: WNK lysine deficient protein kinase 2
• NCBI Official Symbol: WNK2
• NCBI Official Synonym Symbols: PRKWNK2; NY-CO-43; SDCCAG43; P/OKcl.13
• NCBI Protein Information: serine/threonine-protein kinase WNK2
• UniProt Protein Name: Serine/threonine-protein kinase WNK2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: WNK2
• UniProt Synonym Gene Names: KIAA1760; PRKWNK2; SDCCAG43
• UniProt Entry Name: WNK2_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic serine-threonine kinase that belongs to the protein kinase superfamily. The protein plays an important role in the regulation of electrolyte homeostasis, cell signaling survival, and proliferation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

WNK2: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; Wnk family

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; ATP binding

Biological Process: negative regulation of cell proliferation; protein amino acid autophosphorylation; protein amino acid phosphorylation